1887

Abstract

A new cultivation method was successfully applied for the isolation of a hitherto uncultured spiral species associated with ulceration of the non-glandular stomach and gastritis in pigs and formerly described as ‘ Helicobacter suis’. Three isolates, HS1, HS2 and HS3, were subcultured from the stomach mucosa of three pigs after slaughter and were analysed using a polyphasic taxonomic approach. The novel isolates grew on biphasic culture plates or very moist agar bases in microaerobic conditions and exhibited urease, oxidase and catalase activities. Sequencing of the 16S rRNA gene, the 23S rRNA gene, the partial gene and partial genes confirmed that the strains present in the gastric mucosa of pigs constituted a separate taxon, corresponding to ‘’ type 1 strains as detected in the gastric mucosa of humans and other primates. For all genes sequenced, the highest sequence similarities were obtained with and , species isolated from the gastric mucosa of dogs and cats, which have also been detected in the human gastric mucosa and which are commonly referred to as ‘’ type 2. SDS-PAGE of whole-cell proteins of strains HS1, HS2 and HS3 differentiated them from other species of gastric origin. The results of the polyphasic taxonomic analysis confirmed that the novel isolates constitute a novel taxon corresponding to ‘’ type 1 strains from humans and to ‘ H. suis’ from pigs. The name sp. nov. is proposed for the novel isolates with the type strain HS1 (=LMG 23995=DSM 19735).

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2008-06-01
2024-03-28
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Distance matrix analyses for the 16S rRNA, partial , 23S rRNA and partial gene sequences from strains. [ Combined PDF] 177 KB



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