1887

Abstract

In the course of a study of the prokaryotic diversity of a landfill site in Chandigarh, India, a strain designated SK 55 was isolated and characterized using a polyphasic approach. Its 16S rRNA gene sequence showed closest similarity (98.3 %) to that of CMS 21w. The sequence similarity to strains of other hitherto described species of was less than 95.5 %. Strain SK 55 contains peptidoglycan of the A4 type (-Lys–-Asp), MK-8 and MK-7 as the major menaquinones and iso-C as the major fatty acid. Strain SK 55, and , the type species of the genus, had some polar lipids in common (diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a phospholipid and an unknown lipid). However, an aminolipid, an aminophospholipid and an unknown lipid found in the former two organisms are similar, though not identical, but quite different from the profile of . The genomic DNA G+C contents of strain SK 55 (46.0 mol%) and CMS 21w (44.0 mol%) are higher than those reported for the majority of species of (36–42 mol%). As revealed by 16S rRNA gene sequence analysis, strain SK 55 and CMS 21w form a clade which is distinct from the clade occupied by other species of . On the basis of phenotypic characteristics including chemotaxonomic data and analysis of the 16S rRNA gene sequence, we conclude that strain SK 55 should be considered as a member of a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is SK 55 (=MTCC7604 =JCM 14041). CMS 21w shows more similarity in its 16S rRNA gene sequence (98.3 %), DNA G+C content and polar lipid profile to strain SK 55 than to DSM 2281. Phylogenetically, it forms a coherent cluster with strain SK 55 which is separate from the cluster. Moreover, iso-C, anteiso-C and C 7 alcohol are the three major fatty acids in both CMS 21w and SK 55. All these data suggest that should be a member of the genus . However, s can be differentiated from SK 55 in several physiological and biochemical characteristics, especially in the patterns of oxidation and acid production from carbohydrates. The genomic relatedness of CMS 21w and strain SK 55 was also very low (18.0 %). It is therefore logical to transfer to the newly created genus as comb. nov. The type strain is CMS 21w (=MTCC4670 =DSM 15428).

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2009-06-01
2019-10-20
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Polar lipid compositions of strain SK 55 , MTCC4670 and DSM 2281 . [PDF](23 KB)

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Two-dimensional total lipid profiles of MTCC4670 (left), strain SK 55 (middle) and DSM 2281 (right) stained with molybdatophosphoric acid. AL, Aminolipid; APL, aminophospholipid; DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL, phospholipid; UL, unknown lipid.

IMAGE

Two-dimensional total lipid profiles of MTCC4670 (left), strain SK 55 (middle) and DSM 2281 (right) stained with molybdatophosphoric acid. AL, Aminolipid; APL, aminophospholipid; DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL, phospholipid; UL, unknown lipid.

IMAGE

Two-dimensional total lipid profiles of MTCC4670 (left), strain SK 55 (middle) and DSM 2281 (right) stained with molybdatophosphoric acid. AL, Aminolipid; APL, aminophospholipid; DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL, phospholipid; UL, unknown lipid.

IMAGE

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