1887

Abstract

Two free-living nitrogen-fixing bacterial strains, N6 and N7, were isolated from corn rhizosphere. A polyphasic taxonomic approach, including morphological characterization, Biolog analysis, DNA–DNA hybridization, and 16S rRNA, and gene sequence analysis, was taken to analyse the two strains. 16S rRNA gene sequence analysis indicated that strains N6 and N7 both belonged to the genus and were closely related to (98.7 and 98.8 % similarity, respectively) and (97.5 and 97.6 % similarity, respectively). DNA–DNA hybridization of strains N6 and N7 showed reassociation values of 48 and 37 %, respectively, with and 43 % with . Sequences of the and genes of both strains showed 99 and ∼95 % similarity, respectively, with those of . Chemotaxonomic characteristics (Q-10 as quinone system, 18 : 17 as major fatty acid) and G+C content of the DNA (67.6 mol%) were also similar to those of members of the genus . Gene sequences and Biolog and fatty acid analysis showed that strains N6 and N7 differed from the closely related species and . On the basis of these results, it is proposed that these nitrogen-fixing strains represent a novel species. The name sp. nov. is suggested, with N7 (=NCCB 100147=LMG 23989) as the type strain.

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2007-12-01
2019-12-08
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