1887

Abstract

The taxonomic position of a novel actinobacterium (designated SBS-12), which was isolated from beach sand on Jeju Island, Republic of Korea, was determined following a polyphasic taxonomic characterization. Cells of the organism were Gram-positive, catalase-positive, oxidase-negative and non-motile rods. The organism contained -diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H) as predominant menaquinone, and had a polar lipid profile containing phosphatidylglycerol and phosphatidylinositol, iso-C as the major fatty acid and a DNA G+C content of 73.1 mol%. A neighbour-joining tree based on 16S rRNA gene sequences showed that the organism occupies a distinct phylogenetic position within the radiation including representatives of the family . High levels of 16S rRNA gene sequence similarity were found with (97.2 %), (96.9 %), (96.6 %) and (96.3 %). The combination of morphological and chemotaxonomic characteristics was consistent with classification in the genus , but the isolate can be differentiated from all species with validly published names by a range of physiological properties. The name sp. nov. is proposed for this novel organism, with the type strain SBS-12 (=JBRI 2003=KCCM 42681=DSM 18965).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65127-0
2007-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/12/2960.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65127-0&mimeType=html&fmt=ahah

References

  1. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  3. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  4. Lawson P. A., Collins M. D., Schumann P., Tindall B. J., Hirsch P., Labrenz M. 2000; New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp.nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23:219–229 [CrossRef]
    [Google Scholar]
  5. Lee S. D. 2007; Nocardioides furvisabuli sp. nov., isolated from black sand. Int J Syst Evol Microbiol 57:35–39 [CrossRef]
    [Google Scholar]
  6. Lee S. D., Kang S.-O., Hah Y. C. 2000; Hongia gen. nov., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol 50:191–199 [CrossRef]
    [Google Scholar]
  7. Prauser H. 1976; Nocardioides , a new genus of the order Actinomycetales . Int J Syst Bacteriol 26:58–65 [CrossRef]
    [Google Scholar]
  8. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  9. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  10. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  11. Yi H., Chun J. 2004a; Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:1295–1299 [CrossRef]
    [Google Scholar]
  12. Yi H., Chun J. 2004b; Nocardioides aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:2151–2154 [CrossRef]
    [Google Scholar]
  13. Yoon J. H., Rhee S.-K., Lee J.-S., Park Y.-H., Lee S. T. 1997; Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. Int J Syst Bacteriol 47:933–938 [CrossRef]
    [Google Scholar]
  14. Yoon J.-H., Kim I.-G., Kang K. H., Oh T.-K., Park Y.-H. 2004; Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. Int J Syst Evol Microbiol 54:71–75 [CrossRef]
    [Google Scholar]
  15. Yoon J.-H., Kim I.-G., Lee M.-H., Oh T.-K. 2005; Nocardioides kribbensis sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 55:1611–1614 [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Lee C.-H., Oh T.-K. 2006; Nocardioides lentus sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 56:271–275 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65127-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65127-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error