1887

Abstract

Two bacterial strains, PC-142 and PC-147, isolated from poultry litter compost, were characterized with respect to their phenetic and phylogenetic characteristics. The isolates were endospore-forming rods that were reddish in colour after Gram staining. They were catalase- and oxidase-positive, were able to degrade starch and gelatin and grew at 15–40 °C and pH 5.5–10.0. The predominant fatty acids were anteiso-C, iso-C and iso-C, the major respiratory quinone was menaquinone MK-7, the cell-wall peptidoglycan was of the A1 type and the G+C content of the DNA was 58 mol%. The 16S rRNA gene sequence analysis and phenetic characterization indicated that these organisms belong to the genus , with SAFN-007 as the closest phylogenetic neighbour (97.5 %). Strains PC-142, PC-147 and SAFN-007 represent a novel lineage within the genus , characterized by a high DNA G+C content (58–63 mol%). The low levels of 16S rRNA gene sequence similarity with respect to other taxa with validly published names and the identification of distinctive phenetic features in the two isolates indicate that strains PC-142 and PC-147 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PC-147 (=DSM 18784 =NBRC 102415 =LMG 23886).

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2007-10-01
2019-12-15
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vol. , part 10, pp. 2267 - 2271

RAPD patterns.

Extended neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, showing the nearest neighbours of strains PC-142 and PC-147 within the genus .

[PDF file of Supplementary Figs S1 and S2](111 KB)



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