Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 1

sp. nov., isolated from human amniotic fluid Free

Abstract

Two Gram-negative, anaerobic, non-spore-forming, rod-shaped organisms were isolated from human amniotic fluid. Based on morphological and biochemical criteria, the strains were tentatively identified as but they did not appear to correspond to any recognized species of this family. Comparative 16S rRNA gene sequencing studies showed the strains were highly related to each other and confirmed their placement in the genus , but sequence divergence values of >4 % with reference species demonstrated that the organisms from human clinical sources represent a novel species. Phylogenetic analysis revealed the novel organism to be most closely related to , an organism frequently associated with pelvic inflammatory diseases. The major long-chain cellular fatty acids of the novel species consist of iso-C, anteiso-C, iso-C, C, iso-C and iso-3-OH-C. Based on biochemical criteria and phylogenetic considerations, it is proposed that the unknown isolates from human amniotic fluid be assigned to a new species of the genus , as sp. nov. The type strain of is CCUG 53648 (=JCM 14753).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65118-0
2008-01-01
2025-03-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/1/89.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65118-0&mimeType=html&fmt=ahah

References

  1. Brook, I. & Frazier, E. H.(1997). The aerobic and anaerobic bacteriology of perirectal abscesses. J Clin Microbiol 35, 2974–2976. [Google Scholar]
  2. Downes, J., Sutcliffe, I., Tanner, A. C. R. & Wade, W. G.(2005).Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 55, 1551–1555.[CrossRef] [Google Scholar]
  3. Fredricks, D. N., Fiedler, T. L. & Marrazzo, J. M.(2005). Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 353, 1899–1911.[CrossRef] [Google Scholar]
  4. Holdeman, L. V., Cato, E. P. & Moore, W. E. C.(1977).Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  5. Hyman, R. W., Fukushima, M., Diamond, L., Kumm, J., Giudice, L. C. & Davis, R. W.(2005). Microbes on the human vaginal epithelium. Proc Natl Acad Sci U S A 102, 7952–7957.[CrossRef] [Google Scholar]
  6. Mikamo, H., Sato, Y., Hayasaki, Y., Kawazoe, K., Izumi, K., Satoh, M., Kai, J. & Tamaya, T.(1999). In vitro bactericidal activities of antimicrobial agents and morphologic changes on Prevotella bivia. Chemotherapy 45, 342–348.[CrossRef] [Google Scholar]
  7. Nicholas, K. B., Nicholas, H. B., Jr & Deerfield, D. W., II(1997). GeneDoc: analysis and visualization of genetic variation. EMBnet News 4 (2), 1–4. www.embnet.org/files/shared/embnetnews/embnet_news_4_2.pdf [Google Scholar]
  8. Page, R. D. M.(1996). TreeView: an application to display phylogenetic trees on a personal computer. Comput Appl Biosci 12, 357–358. [Google Scholar]
  9. Pearson, W. R. & Lipman, D. J.(1988). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef] [Google Scholar]
  10. Pot, B., Vandamme, P. & Kersters, K.(1994). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  11. Puapermpoonsiri, S., Watanabe, K., Kato, N. & Ueno, K.(1997).In vitro activities of 10 antimicrobial agents against bacterial vaginosis-associated anaerobic isolates from pregnant Japanese and Thai women. Antimicrob Agents Chemother 41, 2297–2299. [Google Scholar]
  12. Saitou, N. & Nei, M.(1987). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  13. Sakamoto, M., Suzuki, M., Huang, Y., Umeda, M., Ishikawa, I. & Benno, Y.(2004).Prevotella shahii sp. nov. and Prevotella salivae sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 54, 877–883.[CrossRef] [Google Scholar]
  14. Sakamoto, M., Huang, Y., Umeda, M., Ishikawa, I. & Benno, Y.(2005a).Prevotella multiformis sp. nov., isolated from human subgingival plaque. Int J Syst Evol Microbiol 55, 815–819.[CrossRef] [Google Scholar]
  15. Sakamoto, M., Umeda, M., Ishikawa, I. & Benno, Y.(2005b).Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque. Int J Syst Evol Microbiol 55, 1839–1843.[CrossRef] [Google Scholar]
  16. Shah, H. N. & Collins, M. D.(1990).Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int J Syst Bacteriol 40, 205–208.[CrossRef] [Google Scholar]
  17. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA–DNA reassociation and 16S rDNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65118-0
Loading
Prevotella amnii sp. nov., isolated from human amniotic fluid
Int J Syst Evol Microbiol 58, 89 (2008); https://doi.org/10.1099/ijs.0.65118-0
/content/journal/ijsem/10.1099/ijs.0.65118-0
/content/journal/ijsem/10.1099/ijs.0.65118-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error