1887

Abstract

A Gram-negative, non-motile, coccobacilli-shaped bacterium, DS-48, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain DS-48 grew optimally at 25 °C and pH 7.0–8.0 in the presence of 0.5 % (w/v) NaCl. It contained Q-10 as the predominant ubiquinone and C 7 and C 2-OH as the major fatty acids. The DNA G+C content was 69.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-48 fell within the genus , clustering with TH-G33 (at a bootstrap confidence level of 100 %). The levels of similarity between the 16S rRNA gene sequence of strain DS-48 and those of the type strains of recognized species were in the range 93.2–98.0 %. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness of DS-48, revealed that this strain differs from recognized species. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain DS-48 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is DS-48 (=KCTC 12874=JCM 14592).

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2007-11-01
2019-10-16
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References

  1. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Gallego, V., Sánchez-Porro, C., García, M. T. & Ventosa, A. ( 2006; ). Roseomonas aquatica sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56, 2291–2295.[CrossRef]
    [Google Scholar]
  4. Han, X. Y., Pham, A. S., Tarrand, J. J., Rolston, K. V., Helsel, L. O. & Levett, P. N. ( 2003; ). Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov. Am J Clin Pathol 120, 256–264.[CrossRef]
    [Google Scholar]
  5. Helsel, L. O., Hollis, D. G., Steigerwalt, A. G. & Levett, P. N. ( 2006; ). Reclassification of Roseomonas fauriae Rihs et al. 1998 as a later heterotypic synonym of Azospirillum brasilense Tarrand et al. 1979. Int J Syst Evol Microbiol 56, 2753–2755.[CrossRef]
    [Google Scholar]
  6. Jiang, C.-Y., Dai, X., Wang, B.-J., Zhou, Y.-G. & Liu, S.-J. ( 2006; ). Roseomonas lacus sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56, 25–28.[CrossRef]
    [Google Scholar]
  7. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  8. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  9. Rihs, J. D., Brenner, D. J., Weaver, R. E., Steigerwalt, A. G., Hollis, D. G. & Yu, V. L. ( 1993; ). Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol 31, 3275–3283.
    [Google Scholar]
  10. Sasser, M. ( 1990; ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Technical Note 101. Newark, DE: MIDI.
  11. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  12. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  13. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  14. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  15. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  16. Yoon, J.-H., Kim, I.-G., Shin, D.-Y., Kang, K. H. & Park, Y.-H. ( 2003; ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef]
    [Google Scholar]
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vol. , part 11, pp. 2485 - 2488

Phenotypic characteristics of strain DS-48 and species [PDF](52 KB)



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