1887

Abstract

A Gram-positive and catalase-negative coccus that formed chains, strain FP15-1, isolated from fermented tea leaves (‘miang’), was studied systematically. The strain was facultatively anaerobic and produced -lactic acid from glucose. Demethylmenaquinone (DMK-7) was the major menaquinone. Straight-chain unsaturated fatty acids C and C were the dominant components. The DNA G+C content was 37.8 mol%. On the basis of 16S rRNA and RNA polymerase subunit () gene sequence analysis, strain FP15-1 was closely related to KCTC 5373, with 99.2 and 93.8 % similarity, respectively. The strain could be clearly distinguished from ATCC 5373 by low DNA–DNA relatedness (≤33.8 %) and phenotypic characteristics. Therefore, this strain represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FP15-1 (=KCTC 13133 =NBRC 101868 =NRIC 0105 =TISTR 932 =PCU 277).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65109-0
2007-09-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/9/2151.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65109-0&mimeType=html&fmt=ahah

References

  1. Baele, M., Baele, P., Vaneechoutte, M., Storms, V., Butaye, P., Devriese, L. A., Verschraegen, G., Gillis, M. & Haesebrouck, F. ( 2000; ). Application of tRNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 38, 4201–4207.
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Collins, M. D. & Jones, D. ( 1979; ). The distribution of isoprenoid quinones in streptococci of serological groups D and N. J Gen Microbiol 114, 27–33.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  5. Ennahar, S. & Cai, Y. ( 2005; ). Biochemical and genetic evidence for the transfer of Enterococcus solitarius Collins et al. 1989 to the genus Tetragenococcus as Tetragenococcus solitarius comb. nov. Int J Syst Evol Microbiol 55, 589–592.[CrossRef]
    [Google Scholar]
  6. Euzéby, J. P. ( 1997; ). List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47, 590–592.[CrossRef]
    [Google Scholar]
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. Fortina, M. G., Ricci, G., Mora, D. & Manachini, P. L. ( 2004; ). Molecular analysis of artisanal Italian cheeses reveals Enterococcus italicus sp. nov. Int J Syst Evol Microbiol 54, 1717–1721.[CrossRef]
    [Google Scholar]
  10. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  11. Hardie, J. M. & Whiley, R. A. ( 1997; ). Classification and overview of the genera Streptococcus and Enterococcus. J Appl Microbiol 83 (Suppl. 1). 1S–11S.[CrossRef]
    [Google Scholar]
  12. Hucker, G. J. & Conn, H. J. ( 1923; ). Method of Gram staining. N Y State Agric Exp Stn Tech Bull 93, 3–37.
    [Google Scholar]
  13. Ikemoto, S., Katoh, K. & Komagata, K. ( 1978; ). Cellular fatty acid composition in methanol-utilizing bacteria. J Gen Appl Microbiol 24, 41–49.[CrossRef]
    [Google Scholar]
  14. Kihara, H. & Snell, E. E. ( 1960; ). Peptides and bacterial growth. J Biol Chem 235, 1409–1414.
    [Google Scholar]
  15. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  16. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  17. Manero, A. & Blanch, A. B. ( 1999; ). Identification of Enterococcus spp. with a biochemical key. Appl Environ Microbiol 65, 4425–4430.
    [Google Scholar]
  18. Merquior, V. L. C., Peralta, J. M., Facklam, R. R. & Teixeira, L. M. ( 1994; ). Analysis of electrophoretic whole-cell protein profiles as a tool for characterization of Enterococcus species. Curr Microbiol 28, 149–153.[CrossRef]
    [Google Scholar]
  19. Naser, S. M., Thompson, F. L., Hoste, B., Gevers, D., Dawyndt, P., Vancanneyt, M. & Swings, J. ( 2005; ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151, 2141–2150.[CrossRef]
    [Google Scholar]
  20. Naser, S. M., Vancanneyt, M., Hoste, B., Snauwaert, C., Vandemeulebroecke, K. & Swings, J. ( 2006; ). Reclassification of Enterococcus flavescens Pompei et al. 1992 as a later synonym of Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984 and Enterococcus saccharominimus Vancanneyt et al. 2004 as a later synonym of Enterococcus italicus Fortina et al. 2004. Int J Syst Evol Microbiol 56, 413–416.[CrossRef]
    [Google Scholar]
  21. Okada, S., Toyoda, T. & Kozaki, M. ( 1978; ). An easy method for the determination of the optical types of lactic acid produced by lactic acid bacteria. Agric Biol Chem 42, 1781–1783.[CrossRef]
    [Google Scholar]
  22. Perrière, G. & Gouy, M. ( 1996; ). WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78, 364–369.[CrossRef]
    [Google Scholar]
  23. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  26. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H. ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef]
    [Google Scholar]
  27. Tanasupawat, S., Ezaki, T., Suzuki, K., Okada, S., Komagata, K. & Kozaki, M. ( 1992; ). Characterization and identification of Lactobacillus pentosus and Lactobacillus plantarum strains from fermented foods in Thailand. J Gen Appl Microbiol 38, 121–134.[CrossRef]
    [Google Scholar]
  28. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  29. Vancanneyt, M., Zamfir, M., Devriese, L. A., Lefebvre, K., Engelbeen, K., Vandemeulebroecke, K., Amar, M., De Vuyst, L., Haesebrouck, F. & Swings, J. ( 2004; ). Enterococcus saccharominimus sp. nov., from dairy products. Int J Syst Evol Microbiol 54, 2175–2179.[CrossRef]
    [Google Scholar]
  30. Whittenbury, R. ( 1963; ). The use of soft agar in the study of condition affecting the utilization of fermentable substrates by lactic acid bacteria. J Gen Microbiol 32, 375–384.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65109-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65109-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2151 - 2154

Phylogenetic trees based on 16S rRNA gene sequences showing the relationships between strain FP15-1 and related bacterial species, generated by the maximum-likelihood method (Fig. S1) and the maximum-parsimony method (Fig. S2). [PDF](23 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error