RT Journal Article SR Electronic(1) A1 Behrendt, Undine A1 Ulrich, Andreas A1 Spröer, Cathrin A1 Schumann, PeterYR 2007 T1 Chryseobacterium luteum sp. nov., associated with the phyllosphere of grasses JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 8 SP 1881 OP 1885 DO https://doi.org/10.1099/ijs.0.65104-0 PB Microbiology Society, SN 1466-5034, AB Three isolates obtained from grass samples were investigated by means of a polyphasic taxonomic study and were shown to represent a novel species within the genus Chryseobacterium. Comparison of 16S rRNA gene sequences and phenotypic features indicated that the three isolates belonged to a single species. On the basis of 16S rRNA gene sequence analysis, the closest phylogenetic neighbours were Chryseobacterium shigense and Chryseobacterium vrystaatense, which formed a stable cluster with the isolates; this phylogeny was supported by a high bootstrap value and was obtained using different treeing methods. A DNA–DNA hybridization study with the closest neighbour, C. shigense DSM 17126T (98.3 % 16S rRNA gene sequence similarity), clearly demonstrated a separate species status for the grass isolate strain P 456/04T. Comparisons involving physiological properties and whole-cell fatty acid profiles confirmed this result at the phenotypic level. On the basis of these results, strain P 456/04T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium luteum sp. nov. is proposed. The type strain is P 456/04T (=DSM 18605T =LMG 23785T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65104-0