Two novel marine, Gram-negative, non-motile, catalase- and oxidase-positive, aerobic bacteria were isolated from coastal seawater in Tokyo Bay. Analysis of almost-complete 16S rRNA gene sequences showed that the two isolates are members of the genus , sharing highest 16S rRNA gene sequence similarity (96.5 %) with NBRC 16710. The DNA–DNA reassociation values between NBRC 16710 and these isolates were only 10–20 %, in contrast to the high DNA relatedness between the two isolates (89 %). At least 1 % (w/v) NaCl was required for growth. Cellular fatty acid profiles revealed C 7 as the major component and C 3-OH as the major hydroxy fatty acid. Ubiquinone-10 was detected as the major respiratory quinone. The G+C content of the genomic DNA of both strains was 69 mol%. On the basis of DNA–DNA hybridization data and physiological and chemotaxonomic characteristics, it is proposed that these strains should be placed in a novel species, sp. nov. The type strain is KKL-A5 (=NBRC 100637 =CIP 108500); KKL-B9 (=NBRC 100640) is a reference strain.


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