A Gram-positive, aerobic, rod-shaped bacterium (strain Eur1 9.5T) was isolated from a 9-m-deep permafrost sample from the Canadian high Arctic. Strain Eur1 9.5T could not be cultivated in liquid medium and grew over the temperature range 5–37 °C; no growth was observed at 42 °C and only slow growth was observed at 5 °C following 1 month of incubation. Eur1 9.5T grew over the pH range 5.5–8.9 and tolerated NaCl concentrations of 0–0.5 % (w/v). Eur1 9.5T grew heterotrophically on complex carbon substrates and chemolithoautotrophically on inorganic sulfur compounds, as demonstrated by growth on sodium thiosulfate and sulfite as sole electron donors. Eur1 9.5T contained iso-C15 : 0 as the major cellular fatty acid and menaquinone 7 (MK-7) as the major respiratory quinone. The cell-wall peptidoglycan was of type A1γ. The DNA G+C content was 53.1 mol%. The 16S rRNA gene sequence of strain Eur1 9.5T was only distantly related (≤87 % sequence similarity over 1407 bp) to any recognized bacterial species. Based on physiological and phylogenetic analyses, strain Eur1 9.5T is suggested to represent a novel species of a new genus, for which the name Tumebacillus permanentifrigoris gen. nov., sp. nov. is proposed. The type strain of Tumebacillus permanentifrigoris is Eur1 9.5T (=DSM 18773T =JCM 14557T).
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol215, 403–410.[CrossRef][Google Scholar]
An, S.-Y., Asahara, M., Goto, K., Kasai, H. & Yokota, A.(2007).Virgibacillus halophilus sp. nov., spore-forming bacteria isolated from soil in Japan. Int J Syst Evol Microbiol57, 1607–1611.[CrossRef][Google Scholar]
Atlas, R. M.(1993).Handbook of Microbiological Media. Boca Raton, FL: CRC Press.
Cole, J. R., Chai, B., Farris, R. J., Wang, Q., Kulam-Syed-Mohideen, A. S., McGarrell, D. M., Bandela, A. M., Cardenas, E., Garrity, G. M. & Tiedje, J. M.(2007). The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucleic Acids Res35, D169–D172.[CrossRef][Google Scholar]
Gonzalez, J. M. & Saiz-Jimenez, C.(2002). A fluorometric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol4, 770–773.[CrossRef][Google Scholar]
Goto, K., Mochida, K., Asahara, M., Suzuki, M., Kasai, H. & Yokota, A.(2003).Alicyclobacillus pomorum sp. nov., a novel thermo-acidophilic, endospore-forming bacterium that does not possess ω-alicyclic fatty acids, and emended description of the genus Alicyclobacillus. Int J Syst Evol Microbiol53, 1537–1544.[CrossRef][Google Scholar]
Goto, K., Mochida, K., Kato, Y., Asahara, M., Fujita, R., An, S. Y., Kasai, H. & Yokota, A.(2007). Proposal of six species of moderately thermophilic, acidophilic, endospore-forming bacteria: Alicyclobacillus contaminans sp. nov., Alicyclobacillus fastidiosus sp. nov., Alicyclobacillus kakegawensis sp. nov., Alicyclobacillus macrosporangiidus sp. nov., Alicyclobacillus sacchari sp. nov. and Alicyclobacillus shizuokensis sp. nov. Int J Syst Evol Microbiol57, 1276–1285.[CrossRef][Google Scholar]
Heyndrickx, M., Vandemeulebroecke, K., Scheldeman, P., Kersters, K., De Vos, P., Logan, N. A., Aziz, A. M., Ali, N. & Berkeley, R. C. W.(1996). A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and of P. peoriae. Int J Syst Bacteriol46, 988–1003.[CrossRef][Google Scholar]
Heyrman, J., Logan, N. A., Busse, H.-J., Balcaen, A., Lebbe, L., Rodriguez-Diaz, M., Swings, J. & de Vos, P.(2003).Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol53, 501–511.[CrossRef][Google Scholar]
Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
Karavaiko, G. I., Bogdanova, T. I., Tourova, T. P., Kondrat'eva, T. F., Tsaplina, I. A., Egorova, M. A., Krasil'nikova, E. N. & Zakharchuk, L. M.(2005). Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus. Int J Syst Evol Microbiol55, 941–947.[CrossRef][Google Scholar]
Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S.(1955). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc77, 4844–4846.[CrossRef][Google Scholar]
Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K.(1997). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol47, 289–298.[CrossRef][Google Scholar]
Steven, B., Léveillé, R., Pollard, W. H. & Whyte, L. G.(2006). Microbial ecology and biodiversity in permafrost. Extremophiles10, 259–267.[CrossRef][Google Scholar]
Steven, B., Briggs, G., McKay, C. P., Pollard, W. H., Greer, C. W. & Whyte, L. G.(2007). Characterization of the microbial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods. FEMS Microbiol Ecol59, 513–523.[CrossRef][Google Scholar]
Tamura, K. & Nei, M.(1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol10, 512–526.
[Google Scholar]
Vali, H., Weiss, B., Li, Y.-L., Sears, S. K., Kim, S. S., Kirschvink, J. L. & Zhang, C. L.(2004). Formation of tabular single-domain magnetite induced by Geobacter metallireducens GS-15. Proc Natl Acad Sci U S A101, 16121–16126.[CrossRef][Google Scholar]