1887

Abstract

A novel yellow-pigmented actinobacterium was isolated from seawater collected from Hwasun Beach in Jeju, Republic of Korea. A comparative analysis of the 16S rRNA gene sequence indicated that the organism, designated HFW-26, was closely related to members of the genus . As found for other species of the genus , strain HFW-26 possessed -diaminopimelic acid as the diagnostic cell-wall diamino acid, contained MK-8(H) as the major menaquinone, contained polar lipids that included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid, and had anteiso-C and anteiso-C as the predominant fatty acids. The G+C content of the DNA was 71.4 mol%. The phylogenetically closest relative was DSM 16132 (99.0 % 16S rRNA gene sequence similarity). However, DNA–DNA hybridization of strain HFW-26 showed 35.1–43.7 % relatedness with respect to DSM 16132. The novel isolate could be clearly distinguished from DSM 16132 on the basis of some cultural, physiological and biochemical characteristics. A battery of phenotypic and genetic data obtained in this study suggest that strain HFW-26 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HFW-26 (=JBRI 2001=KCTC 19221=DSM 18964).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65099-0
2008-02-01
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/500.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65099-0&mimeType=html&fmt=ahah

References

  1. Breed, R. S. ( 1953; ). The families developed from Bacteriaceae Cohn with a description of the family Brevibacteriaceae. Riass Commun VI Congr Int Microbiol Roma 1, 10–15.
    [Google Scholar]
  2. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Jones, D., Keddie, R. M. & Sneath, P. H. A. ( 1980; ). Reclassification of Chromobacterium iodinum (Davis) in a redefined genus Brevibacterium (Breed) as Brevibacterium iodinum nom. rev., comb. nov. J Gen Microbiol 120, 1–10.
    [Google Scholar]
  4. Collins, M. D., Farrow, J. A. E., Goodfellow, M. & Minnikin, D. E. ( 1983; ). Brevibacterium casei sp. nov. and Brevibacterium epidermidis sp. nov. Syst Appl Microbiol 4, 388–395.[CrossRef]
    [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Euzéby, J. P. & Tindall, B. J. ( 2004; ). Status of strains that contravene Rules 27(3) and 30 of the Bacteriological Code. Request for an Opinion. Int J Syst Evol Microbiol 54, 293–301.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. Gavrish, E., Krauzova, V. I., Potekhina, N. V., Karasev, S. G., Plotnikova, E. G., Altyntseva, O. V., Korosteleva, L. A. & Evtushenko, L. I. ( 2004; ). Three new species of brevibacteria, Brevibacterium antiquum sp. nov., Brevibacterium aurantiacum sp. nov., and Brevibacterium permense sp. nov. Microbiology English translation of Mikrobiologiia 73, 176–183.[CrossRef]
    [Google Scholar]
  10. Heyrman, J., Verbeeren, J., Schumann, P., Devos, J., Swings, J. & Vos, P. D. ( 2004; ). Brevibacterium picturae sp. nov., isolated from a damaged mural painting at the Saint-Catherine chapel (Castle Herberstein, Austria). Int J Syst Evol Microbiol 54, 1537–1541.[CrossRef]
    [Google Scholar]
  11. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  12. Ivanova, E. P., Christen, R., Alexeeva, Y. V., Zhukova, N. V., Gorshkova, N. M., Lysenko, A. M., Mikhailov, V. V. & Nicolau, D. V. ( 2004; ). Brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga. Int J Syst Evol Microbiol 54, 2107–2111.[CrossRef]
    [Google Scholar]
  13. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  14. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  15. Küster, E. & Williams, S. T. ( 1964; ). Selection of media for isolation of streptomycetes. Nature 202, 928–929.[CrossRef]
    [Google Scholar]
  16. Lee, S. D. ( 2006; ). Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment. Int J Syst Evol Microbiol 56, 1889–1892.[CrossRef]
    [Google Scholar]
  17. McBride, M. E., Ellner, K. M., Black, H. S., Clarridge, J. E. & Wolf, J. E. ( 1993; ). A new Brevibacterium sp. isolated from infected genital hair of patients with white piedra. J Med Microbiol 39, 255–261.[CrossRef]
    [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  19. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1977; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88, 200–204.
    [Google Scholar]
  20. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  21. Pascual, C. & Collins, M. D. ( 1999; ). Brevibacterium avium sp. nov., isolated from poultry. Int J Syst Bacteriol 49, 1527–1530.[CrossRef]
    [Google Scholar]
  22. Pascual, C., Collins, M. D., Funke, G. & Pitcher, D. G. ( 1996; ). Phenotypic and genotypic characterization of two Brevibacterium strains from the human ear: description of Brevibacterium otitidis sp. nov. Med Microbiol Lett 5, 113–123.
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  25. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  26. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  27. Wauters, G., Charlier, J., Janssens, M. & Delmée, M. ( 2001; ). Brevibacterium paucivorans sp. nov., from human clinical specimens. Int J Syst Evol Microbiol 51, 1703–1707.[CrossRef]
    [Google Scholar]
  28. Wauters, G., Avesani, V., Laffineur, K., Charlier, J., Janssens, M., Van Bosterhaut, B. & Delmée, M. ( 2003; ). Brevibacterium lutescens sp. nov., from human and environmental samples. Int J Syst Evol Microbiol 53, 1321–1325.[CrossRef]
    [Google Scholar]
  29. Wauters, G., Haase, G., Avesani, V., Charlier, J., Janssens, M., Broeck, J. V. & Delmée, M. ( 2004; ). Identification of a novel Brevibacterium species isolated from humans and description of Brevibacterium sanguinis sp. nov. J Clin Microbiol 42, 2829–2832.[CrossRef]
    [Google Scholar]
  30. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65099-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65099-0
Loading

Data & Media loading...

vol. , part 2, pp. 500 - 504

Transmission electron micrographs of cells of strain HFW-26 . [ PDF] 37 KB



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error