Strains of a novel anaerobic, Gram-negative coccus were isolated from the supra-gingival plaque of children. Independent strains from each of six subjects were shown, at a phenotypic level and based on 16S rRNA gene sequencing, to be members of the genus . Analysis revealed that the six strains shared 99.7 % similarity in their 16S rRNA gene sequences and 99.0 % similarity in their gene sequences. The six novel strains formed a distinct group and could be clearly separated from recognized species of the genus of human or animal origin. The novel strains exhibited 98 and 91 % similarity to partial 16S rRNA and gene sequences of ATCC 10790, the most closely related member of the genus. The six novel strains could be differentiated from recognized species of the genus based on partial 16S rRNA and gene sequencing. The six novel strains are thus considered to represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is CF100 (=CCUG 54233=DSM 18960).


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  1. Beighton, D., Hardie, J. M. & Whiley, R. A.(1991). A scheme for the identification of viridans streptococci. J Med Microbiol 35, 367–372.[CrossRef] [Google Scholar]
  2. Byun, R., Carlier, J.-P., Jacques, N. A., Marchandin, H. & Hunter, N.(2007).Veillonella denticariosi sp. nov., isolated from human carious dentine. Int J Syst Evol Microbiol 57, 2844–2848.[CrossRef] [Google Scholar]
  3. Delwiche, E. A., Pestka, J. J. & Tortorello, M. L.(1985). The veillonellae: gram-negative cocci with a unique physiology. Annu Rev Microbiol 39, 175–193.[CrossRef] [Google Scholar]
  4. Jumas-Bilak, E., Carlier, J. P., Jean-Pierre, H., Teyssier, C., Gay, B., Campos, J. & Marchandin, H.(2004).Veillonella montpellierensis sp. nov., a novel, anaerobic, Gram-negative coccus isolated from human clinical samples. Int J Syst Evol Microbiol 54, 1311–1316.[CrossRef] [Google Scholar]
  5. Kolenbrander, P. E. & Moore, L. V. H.(1992). The genus Veillonella. In The Prokaryotes, 2nd edn, pp. 2034–2047. Edited by A. Balows, H. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
  6. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  7. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  8. Marchandin, H., Jean-Pierre, H., Carrière, C., Canovas, F., Darbas, H. & Jumas-Bilak, E.(2001). Prosthetic joint infection due to Veillonella dispar. Eur J Clin Microbiol Infect Dis 20, 340–342. [Google Scholar]
  9. Marchandin, H., Teyssier, C., Simeon De Buochberg, M., Jean-Pierre, H., Carriere, C. & Jumas-Bilak, E.(2003). Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy. Microbiology 149, 1493–1501.[CrossRef] [Google Scholar]
  10. Marchandin, H., Teyssier, C., Jumas-Bilak, E., Robert, M., Artigues, A. C. & Jean-Pierre, H.(2005). Molecular identification of the first human isolate belonging to the Veillonella rattiVeillonella criceti group based on 16S rRNA and dnaK gene sequencing. Res Microbiol 156, 603–607.[CrossRef] [Google Scholar]
  11. Mays, T. D., Holdeman, L. V., Moore, W. E. C., Rogosa, M. & Johnson, J. L.(1982). Taxonomy of the genus Veillonella Prévot. Int J Syst Bacteriol 32, 28–36.[CrossRef] [Google Scholar]
  12. Rogosa, M.(1956). A selective medium for the isolation and enumeration of the veillonella from the oral cavity. J Bacteriol 72, 533–536. [Google Scholar]
  13. Rogosa, M.(1984). Anaerobic Gram-negative cocci. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 680–685. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]

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