1887

Abstract

Gram-positive, rod-shaped, endospore-forming, thermophilic bacteria were isolated from a geothermal soil collected on the north-west slope of Mount Melbourne in Antarctica. They grew aerobically at 42–60 °C (optimum 55 °C) and at pH 4.5–7.5 (optimum pH 5.5). Phylogenetic analysis of 16S rRNA gene sequences showed that these isolates were related most closely to the type strain of (91 % similarity). Growth occurred in the presence of ferrous iron at micromolar concentrations and acid was produced from various sugars. Iso-branched fatty acids C (45.56 %) and C (35.81 %) were the most abundant cellular fatty acids. The DNA G+C content was 55.1 mol%. On the basis of phenotypic and phylogenetic characteristics, it is concluded that these strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MP4 (=CIP 109385 =NCIMB 14276).

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2008-01-01
2019-09-16
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References

  1. Albuquerque, L., Rainey, F. A., Chung, A. P., Sunna, A., Nobre, M. F., Grote, R., Antranikian, G. & De Costa, M. S. ( 2000; ). Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores. Int J Syst Evol Microbiol 50, 451–457.[CrossRef]
    [Google Scholar]
  2. Allan, R. N., Lebbe, L., Heyrman, J., De Vos, P., Buchanan, C. J. & Logan, N. A. ( 2005; ). Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica. Int J Syst Evol Microbiol 55, 1039–1050.[CrossRef]
    [Google Scholar]
  3. Bargagli, R., Skotnicki, M. L., Marri, L., Pepi, M., Mackenzie, A. & Agnorelli, C. ( 2004; ). New record of moss and thermophilic bacteria species and physico-chemical properties of geothermal soils on the northwest slope of Mt. Melbourne (Antarctica). Polar Biol 27, 423–431.[CrossRef]
    [Google Scholar]
  4. Cole, J. R., Chai, B., Marsh, T. L., Farris, R. J., Wang, Q., Kulam, S. A., Chandra, S., McGarrell, D. M., Schmidt, T. M. & other authors ( 2003; ). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  5. Deinhard, G., Saar, J., Krischke, W. & Poralla, K. ( 1987; ). Bacillus cycloheptanicus sp. nov., a new thermophile containing ω-cycloheptane fatty acids. Syst Appl Microbiol 10, 68–73.[CrossRef]
    [Google Scholar]
  6. Goto, K., Matsubara, H., Mochida, K., Matsumura, T., Hara, Y., Niwa, M. & Yamasato, K. ( 2002; ). Alicyclobacillus herbarius sp. nov., a novel bacterium containing omega-cycloheptane fatty acids, isolated from herbal tea. Int J Syst Evol Microbiol 52, 109–113.
    [Google Scholar]
  7. Goto, K., Mochida, K., Asahara, M., Suzuki, M., Kasai, H. & Yokota, A. ( 2003; ). Alicyclobacillus pomorum sp. nov., a novel thermo-acidophilic, endospore-forming bacterium that does not possess ω-alicyclic fatty acids, and emended description of the genus Alicyclobacillus. Int J Syst Evol Microbiol 53, 1537–1544.[CrossRef]
    [Google Scholar]
  8. Goto, K., Mochida, K., Kato, Y., Asahara, M., Fujita, R., An, S. Y., Kasai, H. & Yokota, A. ( 2007; ). Proposal of six species of moderately thermophilic, acidophilic, endospore-forming bacteria: Alicyclobacillus contaminans sp. nov., Alicyclobacillus fastidiosus sp. nov., Alicyclobacillus kakegawensis sp. nov., Alicyclobacillus macrosporangiidus sp. nov., Alicyclobacillus sacchari sp. nov. and Alicyclobacillus shizuokensis sp. nov. . Int J Syst Evol Microbiol 57, 1276–1285.[CrossRef]
    [Google Scholar]
  9. Hiraishi, A., Inagaki, K., Tanimoto, Y., Iwasaki, M., Kishimoto, N. & Tanaka, H. ( 1997; ). Phylogenetic characterization of a new thermoacidophilic bacterium isolated from hot springs in Japan. J Gen Appl Microbiol 43, 295–304.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  11. Karavaiko, G. I., Bogdanova, T. I., Tourova, T. P., Kondrat'eva, T. F., Tsaplina, I. A., Egorova, M. A., Krasil'nikova, E. N. & Zakharchuk, L. M. ( 2005; ). Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus. Int J Syst Evol Microbiol 55, 941–947.[CrossRef]
    [Google Scholar]
  12. Logan, N. A., Lebbe, L., Hoste, B., Goris, J., Forsyth, G., Heyndrick, M., Murray, B. L., Syme, N., Wynn-Williams, D. D. & De Vos, P. ( 2000; ). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich Archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50, 1741–1753.
    [Google Scholar]
  13. Matsubara, H., Goto, K., Matsumura, T., Mochida, K., Iwaki, M., Niwa, M. & Yamasato, K. ( 2002; ). Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, ω-alicyclic fatty acid-containing bacterium isolated from acidic beverages. Int J Syst Evol Microbiol 52, 1681–1685.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Nicolaus, B., Lama, L., Esposito, E., Manca, M. C., Di Prisco, G. & Gambacorta, A. ( 1996; ).Bacillus thermoantarcticus” sp. nov., from Mount Melbourne, Antarctica: a novel thermophilic species. Polar Biol 16, 101–104.
    [Google Scholar]
  16. Nicolaus, B., Improta, R., Manca, M. C., Lama, L., Esposito, E. & Gambacorta, A. ( 1998; ). Alicyclobacilli from an unexplored geothermal soil in Antarctica: Mount Rittmann. Polar Biol 19, 133–141.[CrossRef]
    [Google Scholar]
  17. Poli, A., Esposito, E., Lama, L., Orlando, P., Nicolaus, G., de Appolonia, F., Gambacorta, A. & Nicolaus, B. ( 2006; ). Anoxybacillus amylolyticus sp. nov., a thermophilic amylase producing bacterium isolated from Mount Rittmann (Antarctica). Syst Appl Microbiol 29, 300–307.[CrossRef]
    [Google Scholar]
  18. Powers, E. M. ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758.
    [Google Scholar]
  19. Simbahan, J., Drijber, R. & Blum, P. ( 2004; ). Alicyclobacillus vulcanalis sp. nov., a thermophilic, acidophilic bacterium isolated from Coso Hot Springs, California, USA. Int J Syst Evol Microbiol 54, 1703–1707.[CrossRef]
    [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Bacteriology, pp. 411–442. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Philips. Washington, DC: American Society for Microbiology.
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  22. Tsuruoka, N., Isono, Y., Shida, O., Hemmi, H., Nakayama, T. & Nishino, T. ( 2003; ). Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan. Int J Syst Evol Microbiol 53, 1081–1084.[CrossRef]
    [Google Scholar]
  23. Van de Peer, Y. & De Wachter, R. ( 1997; ). Construction of evolutionary distance trees with treecon for Windows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 13, 227–230.
    [Google Scholar]
  24. Viti, C. & Giovannetti, L. ( 2005; ). Characterization of cultivable heterotrophic bacterial communities in Cr-polluted and unpolluted soils using Biolog and ARDRA approaches. Appl Soil Ecol 28, 101–112.[CrossRef]
    [Google Scholar]
  25. Wisotzkey, J. D., Jurtshuk, P., Jr, Fox, G. E., Deinhard, G. & Poralla, K. ( 1992; ). Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 42, 263–269.[CrossRef]
    [Google Scholar]
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