1887

Abstract

A strain (HBUM 20028) isolated from alkali lake soil in China was studied by a polyphasic taxonomic approach. The strain produced abundant aerial and substrate mycelia. Long spore chains were borne on the aerial mycelium, and the substrate mycelium was often arranged in a shape like a fence or palisade. The special characteristic of strain HBUM 20028 was its abundant growth under alkaline conditions, at pH 8.0–14.0. The cell wall of strain HBUM 20028 contained -diaminopimelic acid but no diagnostic sugar. Major phospholipids included diphosphatidylglycerol and phosphatidylcholine. The major menaquinones were MK-10(H), MK-10(H) and MK-10(H). The major cellular fatty acids were iso-C (31.66 %), anteiso-C (14.85 %) and C 9 (14.73 %). All of these characters consistently indicated that strain HBUM 20028 belongs to the genus . DNA–DNA hybridization between the strain and type strains of related species gave relatedness values far below 70 %. Based on 16S rRNA gene sequence analysis, DNA relatedness and phenotypic characteristics, a novel species with the name sp. nov. is proposed. The type strain is HBUM 20028 (=DSM 45023 =CGMCC 4.2135).

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2008-07-01
2019-10-19
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References

  1. Al-Tai, A. M. & Ruan, J.-S. ( 1994; ). Nocardiopsis halophila sp. nov., a new halophilic actinomycete isolated from soil. Int J Syst Bacteriol 44, 474–478.[CrossRef]
    [Google Scholar]
  2. Al-Zarban, S. S., Abbas, I., Al-Musallam, A. A., Steiner, U., Stackebrandt, E. & Kroppenstedt, R. M. ( 2002; ). Nocardiopsis halotolerans sp. nov., isolated from salt marsh soil in Kuwait. Int J Syst Evol Microbiol 52, 525–529.
    [Google Scholar]
  3. Chun, J., Bae, K. S., Moon, E. Y., Jung, S.-O., Lee, H. K. & Kim, S.-J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  4. Collins, M. D. ( 1985; ). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366.
    [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Evtushenko, L. I., Taran, V. V., Akimov, V. N., Kroppenstedt, R. M., Tiedje, J. M. & Stackebrandt, E. ( 2000; ). Nocardiopsis tropica sp. nov., Nocardiopsis trehalosi sp. nov., nom. rev. and Nocardiopsis dassonvillei subsp. albirubida subsp. nov., comb. nov. Int J Syst Evol Microbiol 50, 73–81.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 2001; ). phylip (phylogeny inference package), version 3.6 (alpha2). Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Grund, E. & Kroppenstedt, R. M. ( 1990; ). Chemotaxonomy and numerical taxonomy of the genus Nocardiopsis Meyer 1976. Int J Syst Bacteriol 40, 5–11.[CrossRef]
    [Google Scholar]
  10. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  11. Holt, J. G., Krieg, N. R. & Sneath, P. H. A. (editors) ( 1994; ). Bergey's Manual of Determinative Bacteriology, 9th edn. Baltimore: Williams & Wilkins.
  12. Hozzein, W. N., Li, W.-J., Ali, M. L. A., Hammouda, O., Mousa, A. S., Xu, L.-H. & Jiang, C.-L. ( 2004; ). Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt. Int J Syst Evol Microbiol 54, 247–252.[CrossRef]
    [Google Scholar]
  13. Kämpfer, P., Busse, H.-J. & Rainey, F. A. ( 2002; ). Nocardiopsis compostus sp. nov., from the atmosphere of a composting facility. Int J Syst Evol Microbiol 52, 621–627.
    [Google Scholar]
  14. Kutchma, A. J., Roberts, M. A., Knaebel, D. B. & Crawford, D. L. ( 1998; ). Small-scale isolation of genomic DNA from Streptomyces mycelia or spores. Biotechniques 24, 452–457.
    [Google Scholar]
  15. Lane, D. J., Pace, B., Olsen, G. J., Stahl, D. A., Sogin, M. L. & Pace, N. R. ( 1985; ). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82, 6955–6959.[CrossRef]
    [Google Scholar]
  16. Lechevalier, H. A. & Lechevalier, M. P. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, pp. 227–291. Special Publication no. 6. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society for Industrial Microbiology.
  17. Li, M.-G., Li, W.-J., Xu, P., Cui, X.-L. & Xu, L.-H. ( 2003; ). Nocardiopsis xinjiangensis sp. nov., a halophilic actinomycete isolated from a saline soil sample in China. Int J Syst Evol Microbiol 53, 317–321.[CrossRef]
    [Google Scholar]
  18. Li, W.-J., Park, D.-J., Tang, S.-K., Wang, D., Lee, J.-C., Xu, L.-H., Kim, C.-J. & Jiang, C.-L. ( 2004; ). Nocardiopsis salina sp. nov., a novel halophilic actinomycete isolated from saline soil in China. Int J Syst Evol Microbiol 54, 1805–1809.[CrossRef]
    [Google Scholar]
  19. Li, W.-J., Kroppenstedt, R. M., Wang, D., Tang, S.-K., Lee, J.-C., Park, D.-J., Kim, C.-J., Xu, L.-H. & Jiang, C.-L. ( 2006; ). Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004. Int J Syst Evol Microbiol 56, 1089–1096.[CrossRef]
    [Google Scholar]
  20. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  21. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  22. Meyer, J. ( 1976; ). Nocardiopsis, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 487–493.[CrossRef]
    [Google Scholar]
  23. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  24. Peltola, J. S. P., Andersson, M. A., Kämpfer, P., Auling, G., Kroppenstedt, R. M., Busse, H.-J., Salkinoja-Salonen, M. S. & Rainey, F. A. ( 2001; ). Isolation of toxigenic Nocardiopsis strains from indoor environments and description of two new Nocardiopsis species, N. exhalans sp. nov. and N. umidischolae sp. nov. Appl Environ Microbiol 67, 4293–4304.[CrossRef]
    [Google Scholar]
  25. Sabry, S. A., Ghanem, N. B., Abu-Ella, G. A., Schumann, P., Stackebrandt, E. & Kroppenstedt, R. M. ( 2004; ). Nocardiopsis aegyptia sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 54, 453–456.[CrossRef]
    [Google Scholar]
  26. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  27. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  28. Schippers, A., Bosecker, K., Willscher, S., Spröer, C., Schumann, P. & Kroppenstedt, R. M. ( 2002; ). Nocardiopsis metallicus sp. nov., a metal-leaching actinomycete isolated from an alkaline slag dump. Int J Syst Evol Microbiol 52, 2291–2295.[CrossRef]
    [Google Scholar]
  29. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  30. Yassin, A. F., Galinski, E. A., Wohlfarth, A., Jahnke, K.-D., Schaal, K. P. & Trüper, H. G. ( 1993; ). A new actinomycete species, Nocardiopsis lucentensis sp. nov. Int J Syst Bacteriol 43, 266–271.[CrossRef]
    [Google Scholar]
  31. Yassin, A. F., Rainey, F. A., Burghardt, J., Gierth, D., Ungerechts, J., Lux, I., Seifert, P., Bal, C. & Schaal, K. P. ( 1997; ). Description of Nocardiopsis synnemataformans sp. nov., elevation of Nocardiopsis alba subsp. prasina to Nocardiopsis prasina comb. nov., and designation of Nocardiopsis antarctica and Nocardiopsis alborubida as later subjective synonyms of Nocardiopsis dassonvillei. Int J Syst Bacteriol 47, 983–988.[CrossRef]
    [Google Scholar]
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