1887

Abstract

A psychrotolerant, obligately alkaliphilic, collagenolytic enzyme-producing bacterium, strain AC40, was isolated from a deep-sea sediment off Torishima Island, Japan, at a depth of 4026 m. Phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium was closely related to members of the genus , with highest sequence similarity (97.9 %) to 1E1. DNA–DNA hybridization experiments of strain AC40 with 1E1 revealed a level of relatedness of less than 30 %. Cells of strain AC40 were strictly aerobic, rod-shaped, Gram-negative and motile by means of a single polar flagellum. The organism grew over a range of temperatures from 5 to 37 °C and at initial pH values between 7.0 and 10.5. Optimal growth was observed at 33 °C and at pH 8.5–10.0. Cellular fatty acids of strain AC40 were predominantly saturated and mono-unsaturated straight-chain components (C and C). The major isoprenoid quinone was Q-8. The G+C content of the DNA was 49.3 mol%. Phylogenetic characteristics, physiological properties and DNA–DNA hybridization data indicate that strain AC40 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AC40 (=JCM 14267=NCIMB 14266).

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2007-07-01
2021-10-24
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