1887

Abstract

A psychrotolerant, obligately alkaliphilic, collagenolytic enzyme-producing bacterium, strain AC40, was isolated from a deep-sea sediment off Torishima Island, Japan, at a depth of 4026 m. Phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium was closely related to members of the genus , with highest sequence similarity (97.9 %) to 1E1. DNA–DNA hybridization experiments of strain AC40 with 1E1 revealed a level of relatedness of less than 30 %. Cells of strain AC40 were strictly aerobic, rod-shaped, Gram-negative and motile by means of a single polar flagellum. The organism grew over a range of temperatures from 5 to 37 °C and at initial pH values between 7.0 and 10.5. Optimal growth was observed at 33 °C and at pH 8.5–10.0. Cellular fatty acids of strain AC40 were predominantly saturated and mono-unsaturated straight-chain components (C and C). The major isoprenoid quinone was Q-8. The G+C content of the DNA was 49.3 mol%. Phylogenetic characteristics, physiological properties and DNA–DNA hybridization data indicate that strain AC40 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AC40 (=JCM 14267=NCIMB 14266).

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2007-07-01
2019-10-22
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References

  1. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  2. Baumann, L., Baumann, P., Mandel, M. & Allen, R. D. ( 1972; ). Taxonomy of aerobic marine eubacteria. J Bacteriol 110, 402–429.
    [Google Scholar]
  3. Bond, M. D. & Van Wart, H. E. ( 1984; ). Characterization of the individual collagenases from Clostridium histolyticum. Biochemistry 23, 3085–3091.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Hisano, T., Abe, S., Wakashiro, M., Kimura, A. & Murata, K. ( 1989; ). Isolation and properties of collagenase with caseinolytic activity from Pseudomonas sp. J Ferment Bioeng 68, 399–403.[CrossRef]
    [Google Scholar]
  6. Horikoshi, K. ( 1999; ). Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev 63, 735–750.
    [Google Scholar]
  7. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66, 22–26.
    [Google Scholar]
  8. Khare, S. D., Krco, C. J., Griffiths, M. M., Luthra, H. S. & David, C. S. ( 1995; ). Oral administration of an immunodominant human collagen peptide modulates collagen-induced arthritis. J Immunol 155, 3653–3659.
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  11. Liu, J., Xue, Y., Wang, Q., Wei, Y., Swartz, T. H., Hicks, D. B., Ito, M., Ma, Y. & Krulwich, T. A. ( 2005; ). The activity profile of the NhaD-type Na+(Li+)/H+ antiporter from the soda lake haloalkaliphile Alkalimonas amylolytica is adaptive for the extreme environment. J Bacteriol 187, 7589–7595.[CrossRef]
    [Google Scholar]
  12. Ma, Y., Xue, Y., Grant, W. D., Collins, N. C., Duckworth, A. W., Van Steenbergen, R. P. & Jones, B. E. ( 2004; ). Alkalimonas amylolytica gen. nov., sp. nov., and Alkalimonas delamerensis gen. nov., sp. nov., novel alkaliphilic bacteria from soda lakes in China and East Africa. Extremophiles 8, 193–200.[CrossRef]
    [Google Scholar]
  13. Minakuchi, Y., Takeshita, F., Kosaka, N., Sasaki, H., Yamamoto, Y., Kouno, M., Honma, K., Nagahara, S., Hanai, K. & other authors ( 2004; ). Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo. Nucleic Acids Res 32, e109.[CrossRef]
    [Google Scholar]
  14. Myers, L. K., Rosloniec, E. F., Seyer, J. M., Stuart, J. M. & Kang, A. H. ( 1993; ). A synthetic peptide analogue of a determinant of type II collagen prevents the onset of collagen-induced arthritis. J Immunol 150, 4652–4658.
    [Google Scholar]
  15. Nakayama, T., Tsuruoka, N., Akai, M. & Nishino, T. ( 2000; ). Thermostable collagenolytic activity of a novel thermophilic isolate, Bacillus sp. strain NTAP-1. J Biosci Bioeng 89, 612–614.[CrossRef]
    [Google Scholar]
  16. Okamoto, M., Yonejima, Y., Tsujimoto, Y., Suzuki, Y. & Watanabe, K. ( 2001; ). A thermostable collagenolytic protease with a very large molecular mass produced by thermophilic Bacillus sp. strain MO-1. Appl Microbiol Biotechnol 57, 103–108.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sasagawa, Y., Izaki, K., Matsubara, Y., Suzuki, K., Kojima, H. & Kamio, Y. ( 1995; ). Molecular cloning and sequence analysis of the gene encoding the collagenase from Cytophaga sp. L43-1 strain. Biosci Biotechnol Biochem 59, 2068–2073.[CrossRef]
    [Google Scholar]
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  21. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Watanabe, K. ( 2004; ). Collagenolytic proteases from bacteria. Appl Microbiol Biotechnol 63, 520–526.[CrossRef]
    [Google Scholar]
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