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A Gram-negative, motile and rod-shaped Sphingomonas-like bacterial strain, DS-28T, was isolated from the soil of Dokdo, Korea, and was subjected to a polyphasic taxonomic study. It grew optimally at around pH 6.5 and 25 °C in the presence of 0–0.5 % NaCl. It contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c, C14 : 0 2-OH and C16 : 0 as the major fatty acids. Major polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 67.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-28T belonged to the genus Sphingomonas. Levels of 16S rRNA gene sequence similarity between strain DS-28T and the type strains of Sphingomonas species were in the range 93.3–98.3 %. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain DS-28T is distinguishable from recognized Sphingomonas species. On the basis of phenotypic, phylogenetic and genetic data, strain DS-28T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas insulae sp. nov. is proposed. The type strain is DS-28T (=KCTC 12872T=JCM 14603T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 1, pp. 231–236
Supplementary Fig. S1. Two-dimensional thin-layer chromatogram of polar lipids of Sphingomonas insulae DS-28 T. [PDF](124 KB)