1887

Abstract

A Gram-negative, non-motile and rod-, oval- or coccoid-shaped bacterial strain, DSW-25, which is phylogenetically closely related to the genera and , was isolated from seawater of the East Sea, Korea, and subjected to a polyphasic taxonomic study. Strain DSW-25 grew optimally at pH 7.0–8.0 and at 25 °C. It contained Q-10 as the predominant ubiquinone and C 7 as the major fatty acid. Major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content was 56.9 mol%. Strain DSW-25 exhibited 16S rRNA gene sequence similarity values of 98.4 % to the type strain of and of 96.6–97.6 % to species. There were no distinct phenotypic, particularly chemotaxonomic, properties to differentiate and strain DSW-25 from the genus . DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain DSW-25 differs from recognized species and . On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain DSW-25 was classified in the genus as a member of a novel species, for which the name sp. nov. is proposed. The type strain is strain DSW-25 (=KCTC 12864 =JCM 14565). It is also proposed that be transferred to the genus as comb. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65071-0
2007-08-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/8/1788.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65071-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes pp 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer-Verlag;
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001 Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
  3. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Ivanova E. P., Gorshkova N. M., Sawabe T., Zhukova N. V., Hayashi K., Kurilenko V. V., Alexeeva Y., Buljan V., Nicolau D. V. other authors 2004 Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish ( Stellaster equestris ) and sea grass ( Zostera marina ). Int J Syst Evol Microbiol 54, 475–480 [CrossRef]
  7. Komagata K., Suzuki K. 1987; Lipids and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  8. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. 1999; Roseovarius tolerans gen. nov., sp. nov. a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49:137–147 [CrossRef]
    [Google Scholar]
  9. Labrenz M., Tindall B. J., Lawson P. A., Collins M. D., Schumann P., Hirsch P. 2000; Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., α -3- Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake. Int J Syst Evol Microbiol 50:303–313 [CrossRef]
    [Google Scholar]
  10. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  11. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  13. Park J. R., Bae J.-W., Nam Y.-D., Chang H.-W., Kwon H.-Y., Quan Z.-X., Park Y.-H. 2007; Sulfitobacter litoralis sp. nov., a marine bacterium isolated from the East Sea. Int J Syst Evol Microbiol 57:692–695 [CrossRef]
    [Google Scholar]
  14. Pukall R., Buntefuß D., Frühling A., Rohde M., Kroppenstedt R. M., Burghardt J., Lebaron P., Bernard L., Stackebrandt E. 1999; Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α - Proteobacteria . Int J Syst Bacteriol 49:513–519 [CrossRef]
    [Google Scholar]
  15. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  16. Sorokin D. Y. 1995 Sulfitobacter pontiacus gen. nov., sp. nov. – a new heterotrophic bacterium from the Black Sea, specialized on sulfite oxidation. Microbiology English translation of Mikrobiologiia ) 64295–305
  17. Sorokin D. Y. 1996; Sulfitobacter pontiacus gen. nov., sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List no. 56. Int J Syst Bacteriol 46:362–363 [CrossRef]
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  19. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  20. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  21. Wagner-Döbler I., Rheims H., Felske A., El-Ghezal A., Flade-Schröder D., Laatsch H., Lang S., Pukall R., Tindall B. J. 2004; Oceanibulbus indolifex gen. nov., sp. nov., a North Sea alphaproteobacterium that produces bioactive metabolites. Int J Syst Evol Microbiol 54:1177–1184 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  24. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Kang S.-J., Lee S.-Y., Lee C.-H., Oh T.-K. 2005a; Maribacter dokdonensis sp. nov., isolated from sea water off a Korean island, Dokdo. Int J Syst Evol Microbiol 55:2051–2055 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Kang S.-J., Lee C.-H., Oh T.-K. 2005b; Dokdonia donghaensis gen. nov., sp. nov. isolated from sea water off a Korean island, Dokdo. Int J Syst Evol Microbiol 55:2323–2328 [CrossRef]
    [Google Scholar]
  28. Yoon J.-H., Kang S.-J., Lee C.-H., Oh T.-K. 2006a; Donghaeana dokdonensis gen. nov., sp. nov. isolated from sea water. Int J Syst Evol Microbiol 56:187–191 [CrossRef]
    [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Oh T.-K. 2006b; Polaribacter dokdonensis sp. nov., isolated from seawater off Dokdo in Korea. Int J Syst Evol Microbiol 56:1251–1255 [CrossRef]
    [Google Scholar]
  30. Yoon J.-H., Kang S.-J., Oh T.-K. 2007; Sulfitobacter marinus sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:302–305 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65071-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65071-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error