1887

Abstract

Strain KV-657 was isolated from a paddy field soil sample collected in Japan using GPM agar plates supplemented with catalase. The strain was a Gram-positive, aerobic organism that formed branching hyphae with -diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major menaquinone was MK-8(H). Mycolic acids were not detected. The G+C content of the DNA was 70 mol%. 16S rRNA gene sequence analysis revealed that this strain is closely related to DSM 43043, with a similarity of 97.6 %. Based on the morphological, biochemical and chemotaxonomic properties of this strain and phylogenetic analysis, it was concluded that this isolate represents a new genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is KV-657 (=NRRL B-24470 =NBRC 101802).

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2007-09-01
2019-10-17
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vol. , part 9, pp. 2163 - 2166

Scanning electron micrographs of cells from a 2-day-old culture of strain KV-657 grown on GPM agar medium at 27 °C showing branching hyphae (a) and fragmentary hyphae (b).

HPLC traces from menaquinone analysis of NBRC 12989 and strain KV-657 .

[PDF file of Supplementary Figs S1 and S2](391 KB)



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