1887

Abstract

Novel halophilic, alkalithermophilic, Gram-type-positive bacterial strains were isolated from sediment of alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Cells of strain JW/NM-WN-LF were rod-shaped, non-spore-forming and non-motile. Strain JW/NM-WN-LF grew (at pH 9.5) between 35 and 56 °C, with an optimum at 53 °C. The pH range for growth was 8.3–10.6, with an optimum at pH 9.5 and no growth at pH 8.2 or below, or at pH 10.8 or above. At the optimum pH and temperature, the strain grew in the Na range of 3.1–4.9 M (1.5–3.3 M added NaCl) and optimally between 3.3 and 3.9 M Na (1.7–2.3 M added NaCl). Strain JW/NM-WN-LF utilized fructose, cellobiose, ribose, trehalose, trimethylamine, pyruvate, Casamino acids, acetate, xylose and peptone as carbon and energy sources. Fumarate (20 mM), SO (20 mM), NO (20 mM) and iron(III) citrate (20 mM) were utilized as electron acceptors. During growth on sucrose, the isolate produced acetate and formate as major fermentation products. Main cellular fatty acids were iso-branched 15 : 0, i17 : 0 dimethylacetal and 16 : 0 dimethylacetal. The G+C content of genomic DNA was 40.4 mol% (HPLC). On the basis of genotypic and phenotypic characteristics, it is proposed that strain JW/NM-WN-LF represents a novel genus and species, gen. nov., sp. nov. The type strain is JW/NM-WN-LF (=DSM 18059=ATCC BAA-1301). Based on 16S rRNA gene sequence analysis, the strain forms a novel lineage within the class ‘’ and clusters with uncultivated bacteria and unidentified strains retrieved from alkaline, hypersaline environments. The phylogenetic data suggest that the lineage represents a novel family, fam. nov., and order, ord. nov.

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2007-11-01
2021-02-25
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