1887

Abstract

Novel halophilic, alkalithermophilic, Gram-type-positive bacterial strains were isolated from sediment of alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Cells of strain JW/NM-WN-LF were rod-shaped, non-spore-forming and non-motile. Strain JW/NM-WN-LF grew (at pH 9.5) between 35 and 56 °C, with an optimum at 53 °C. The pH range for growth was 8.3–10.6, with an optimum at pH 9.5 and no growth at pH 8.2 or below, or at pH 10.8 or above. At the optimum pH and temperature, the strain grew in the Na range of 3.1–4.9 M (1.5–3.3 M added NaCl) and optimally between 3.3 and 3.9 M Na (1.7–2.3 M added NaCl). Strain JW/NM-WN-LF utilized fructose, cellobiose, ribose, trehalose, trimethylamine, pyruvate, Casamino acids, acetate, xylose and peptone as carbon and energy sources. Fumarate (20 mM), SO (20 mM), NO (20 mM) and iron(III) citrate (20 mM) were utilized as electron acceptors. During growth on sucrose, the isolate produced acetate and formate as major fermentation products. Main cellular fatty acids were iso-branched 15 : 0, i17 : 0 dimethylacetal and 16 : 0 dimethylacetal. The G+C content of genomic DNA was 40.4 mol% (HPLC). On the basis of genotypic and phenotypic characteristics, it is proposed that strain JW/NM-WN-LF represents a novel genus and species, gen. nov., sp. nov. The type strain is JW/NM-WN-LF (=DSM 18059=ATCC BAA-1301). Based on 16S rRNA gene sequence analysis, the strain forms a novel lineage within the class ‘’ and clusters with uncultivated bacteria and unidentified strains retrieved from alkaline, hypersaline environments. The phylogenetic data suggest that the lineage represents a novel family, fam. nov., and order, ord. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65068-0
2007-11-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2507.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65068-0&mimeType=html&fmt=ahah

References

  1. Baross, J. A. ( 1998; ). Do the geological and geochemical records of early earth support the prediction from global phylogenetic models of a thermophilic cenancestor? In Thermophiles: The Keys to Molecular Evolution and the Origin of Life, pp. 3–18. Edited by J. Wiegel & M. W. Adams, Philadelphia, PA: Taylor & Francis.
  2. Bligh, E. G. & Dyer, W. J. ( 1959; ). A rapid method of total lipid extraction and purification. Can J Biochem physiol 37, 911–917.[CrossRef]
    [Google Scholar]
  3. Daumas, S., Cord-Ruwisch, R. & Garcia, J. L. ( 1988; ). Desulfotomaculum geothermicum sp. nov., a thermophilic, fatty acid-degrading, sulfate-reducing bacterium isolated with H2 from geothermal ground water. Antonie van Leeuwenhoek 54, 165–178.[CrossRef]
    [Google Scholar]
  4. Doetsch, R. N. ( 1981; ). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Philips, Washington, DC: American Society for Microbiology.
  5. Guckert, J. B., Antworth, C. P., Nichols, P. D. & White, D. C. ( 1985; ). Phospholipid, ester-linked fatty acid profiles as reproducible assays for changes in prokaryotic community structure of estuarine sediments. FEMS Microbiol Lett 31, 147–158.[CrossRef]
    [Google Scholar]
  6. Jones, B. E., Grant, W. D., Duckworth, A. W. & Owenson, G. G. ( 1998; ). Microbial diversity of soda lakes. Extremophiles 2, 191–200.[CrossRef]
    [Google Scholar]
  7. Kevbrin, V. V. & Zavarzin, G. A. ( 1992; ). The effect of sulfur compounds on the growth of the halophilic homoacetic bacterium Acetohalobium arabaticum. Mikrobiologiya 61, 812–817 (in Russian).
    [Google Scholar]
  8. Lee, Y. J., Wagner, I. D., Brice, M. E., Kevbrin, V. V., Mills, G. L., Romanek, C. S. & Wiegel, J. ( 2005; ). Thermosediminibacter oceani gen. nov., sp. nov. and Thermosediminibacter litoriperuensis sp. nov., new anaerobic thermophilic bacteria isolated from Peru Margin. Extremophiles 9, 375–383.[CrossRef]
    [Google Scholar]
  9. Ljungdahl, L. & Wiegel, J. ( 1986; ). Working with anaerobic bacteria. In Manual of Industrial Microbiology and Biotechnology, pp. 84–96. Edited by A. L. Demain & N. A. Solomon. Washington, DC: American Society for Microbiology.
  10. Mesbah, N. M. & Wiegel, J. ( 2006; ). Isolation, cultivation and characterization of alkalithermophiles. Methods Microbiol, 35, 451–468.
    [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. Mesbah, N. M., Abou-El-Ela, S. H. & Wiegel, J. ( 2007; ). Novel and unexpected prokaryotic diversity in water and sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Microb Ecol (in press). doi:10.1007/s00248-006-9193-y
    [Google Scholar]
  13. Nazina, T. N., Rozanova, E., Belyakova, E., Lysenko, A. M., Poltaraus, A., Tourova, T. P., Osipov, G. A. & Belyaev, S. S. ( 2005; ). Description of Desulfotomaculum nigrificans subsp. salinus as a new species, Desulfotomaculum salinum sp. nov. Mikrobiologiia 74, 654–662 (in Russian).
    [Google Scholar]
  14. Oren, A. ( 2000; ). Life at high salt concentrations. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community, 3rd edn, release 3.7. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer-Verlag.
  15. Owenson, G. G. ( 1997; ). Obligately anaerobic alkaliphiles from Kenya soda lake sediments. PhD thesis, University of Leicester, UK.
  16. Peacock, A. D., Mullen, M. D., Ringelberg, D. B., Tyler, D. D., Hedrick, D. B., Gale, P. M. & White, D. C. ( 2001; ). Soil microbial community responses to dairy manure or ammonium nitrate applications. Soil Biol Biochem 33, 1011–1019.[CrossRef]
    [Google Scholar]
  17. Stookey, L. L. ( 1970; ). Ferrozine – a new spectrophotometric reagent for iron. Anal Chem 42, 779–781.[CrossRef]
    [Google Scholar]
  18. Wiegel, J. ( 1981; ). Distinction between the Gram reaction and the Gram type of bacteria. Int J Syst Bacteriol 31, 88 [CrossRef]
    [Google Scholar]
  19. Wiegel, J. ( 1990; ). Temperature spans for growth: hypothesis and discussion. FEMS Microbiol Lett 75, 155–169.[CrossRef]
    [Google Scholar]
  20. Wiegel, J. ( 1998a; ). Anaerobic alkalithermophiles, a novel group of extremophiles. Extremophiles 2, 257–267.[CrossRef]
    [Google Scholar]
  21. Wiegel, J. ( 1998b; ). Lateral gene exchange, an evolutionary mechanism for extending the upper and lower temperature limits for growth of microorganisms? A hypothesis. In Thermophiles: The Keys to Molecular Evolution and the Origin of Life?, pp. 177–185. Edited by J. Wiegel & M. W. Adams. Philadelphia, PA: Taylor & Francis.
  22. Wolin, E. A., Wolin, M. J. & Wolfe, R. S. ( 1963; ). Formation of methane by bacterial extracts. J Biol Chem 238, 2882–2886.
    [Google Scholar]
  23. Zavarzin, G. A. ( 1993; ). Epicontinental soda lakes are probable relict biotopes of terrestrial biota formation. Microbiology English translation of Mikrobiologiya 62, 473–479.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65068-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65068-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2507 – 2512

PLFA composition of strain JW/NM-WN-LF

Dependence of growth rate of strain JW/NM-WN-LF on temperature and medium pH

Fitch–Margoliash tree based on 16S rRNA gene sequences

[ Single PDF file] (1.1 MB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error