@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65067-0, author = "Ommedal, Hege and Torsvik, Terje", title = "Desulfotignum toluenicum sp. nov., a novel toluene-degrading, sulphate-reducing bacterium isolated from an oil-reservoir model column", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2007", volume = "57", number = "12", pages = "2865-2869", doi = "https://doi.org/10.1099/ijs.0.65067-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65067-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-negative, sulphate-reducing bacterium (strain H3T) was isolated from an oil-reservoir model column. The new isolate was able to oxidize toluene coupled to hydrogen sulphide production. For growth, the optimum salt concentration was 1.5 % (w/v), the optimum pH was 7.2 and the optimum temperature was 34 °C. The cells were straight to slightly curved rods, 0.6–1.0 μm in diameter and 1.4–2.5 μm in length. The predominant fatty acids were C16 : 0, C16 : 1 ω7c and C17 : 0 cyclo, and the cells also contained dimethylacetals. Cloning and sequencing of a 1505 bp long fragment of the 16S rRNA gene showed that strain H3T is a member of the Deltaproteobacteria and is related closely to Desulfotignum balticum DSM 7044T. The G+C content of the DNA was 52.0 mol% and the DNA–DNA similarity to D. balticum DSM 7044T was 56.1 %. Based on differences in DNA sequence and the unique property of toluene degradation, it is proposed that strain H3T should be designated a member of a novel species within the genus Desulfotignum, for which the name Desulfotignum toluenicum sp. nov. is proposed. The type strain is H3T (=DSM 18732T=ATCC BAA-1460T).", }