1887

Abstract

A Gram-negative, sulphate-reducing bacterium (strain H3) was isolated from an oil-reservoir model column. The new isolate was able to oxidize toluene coupled to hydrogen sulphide production. For growth, the optimum salt concentration was 1.5 % (w/v), the optimum pH was 7.2 and the optimum temperature was 34 °C. The cells were straight to slightly curved rods, 0.6–1.0 μm in diameter and 1.4–2.5 μm in length. The predominant fatty acids were C, C 7 and C cyclo, and the cells also contained dimethylacetals. Cloning and sequencing of a 1505 bp long fragment of the 16S rRNA gene showed that strain H3 is a member of the and is related closely to DSM 7044. The G+C content of the DNA was 52.0 mol% and the DNA–DNA similarity to DSM 7044 was 56.1 %. Based on differences in DNA sequence and the unique property of toluene degradation, it is proposed that strain H3 should be designated a member of a novel species within the genus , for which the name sp. nov. is proposed. The type strain is H3 (=DSM 18732=ATCC BAA-1460).

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2007-12-01
2024-12-13
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