1887

Abstract

Three Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from clinical specimens between 1992 and 2000. On the basis of 16S rRNA gene sequence similarities, these strains (CCUG 30717, CCUG 43892 and CCUG 38531) were shown to belong to the , most closely related to (99.0 and 98.2 % similarity to the type strain). Chemotaxonomic data (major ubiquinone Q-10; major polyamines spermidine, -homospermidine and putrescine; major polar lipids phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids C 7 and C cyclo 8) supported the affiliation of the isolates to the genus . The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from described species. Isolates CCUG 30717 and CCUG 43892 were closely related on the basis of DNA–DNA reassociation experiments and therefore represent one novel species, for which the name sp. nov. is proposed, with the type strain CCUG 30717 (=CIP 109451). Isolate CCUG 38531 was different from these strains and also from other species. For this strain, the name sp. nov. is proposed, with the type strain CCUG 38531 (=CIP 109452).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65066-0
2007-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2513.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65066-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  2. Auling G., Busse H.-J., Pilz F., Webb L., Kneifel H., Claus D. 1991; Rapid differentiation by polyamine analysis of Xanthomonas strains from phytopathogenic pseudomonads and other members of the class Proteobacteria interacting with plants. Int J Syst Bacteriol 41:223–228 [CrossRef]
    [Google Scholar]
  3. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [CrossRef]
    [Google Scholar]
  4. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47:698–708 [CrossRef]
    [Google Scholar]
  5. Busse H.-J., Hauser E., Kämpfer P. 2005; Description of two novel species, Sphingomonas abaci sp.nov. and Sphingomonas panni sp. nov. Int J Syst Evol Microbiol 55:2565–2569 [CrossRef]
    [Google Scholar]
  6. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Hamana K., Takeuchi M. 1998; Polyamine profiles as chemotaxonomic markers within alpha, beta, gamma, delta, and epsilon subclasses of class Proteobacteria : distribution of 2-hydroxyputrescine and homospermidine. Microbiol Cult Collect 14:1–14
    [Google Scholar]
  8. Holmes B., Popoff M., Kiredjian M., Kersters K. 1988; Ochrobactrum anthropi gen. nov., sp. nov. from human clinical specimens and previously known as group Vd. Int J Syst Bacteriol 38:406–416 [CrossRef]
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Müller C., Mau M., Neef A., Auling G., Busse H.-J., Osborn A. M., Stolz A. 1999; Description of Pseudaminobacter gen. nov. with two new species, Pseudaminobacter salicylatoxidans sp.nov. and Pseudaminobacter defluvii sp. nov. Int J Syst Bacteriol 49:887–897 [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003a; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
    [Google Scholar]
  13. Kämpfer P., Buczolits S., Albrecht A., Busse H.-J., Stackebrandt E. 2003b; Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int J Syst Evol Microbiol 53:893–896 [CrossRef]
    [Google Scholar]
  14. Kämpfer P., Rosselló-Mora R., Scholz H. C., Welinder-Olsson C., Falsen E., Busse H.-J. 2006; Description of Pseudochrobactrum gen. nov., with the two species Pseudochrobactrum asaccharolyticum sp.nov. and Pseudochrobactrumsaccharolyticum sp. nov. Int J Syst Evol Microbiol 56:1823–1829 [CrossRef]
    [Google Scholar]
  15. Kämpfer P., Scholz H., Huber B., Thummes K., Busse H.-J., Maas E. W., Falsen E. 2007; Description of Pseudochrobactrum kiredjianiae sp. nov. Int J Syst Evol Microbiol 57:755–760 [CrossRef]
    [Google Scholar]
  16. Kumar S., Tamura K., Jakobsen I.-B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  17. Lebuhn M., Achouak W., Schloter M., Berge O., Meier H., Barakat M., Hartmann A., Heulin T. 2000; Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp.nov. and Ochrobactrum grignonense sp. nov. Int J Syst Evol Microbiol 50:2207–2223 [CrossRef]
    [Google Scholar]
  18. Lechner U., Baumbach R., Becker D., Kitunen V., Auling G., Salkinoja-Salonen M. 1995; Degradation of 4-chloro-2-methylphenol by an activated sludge isolate and its taxonomic description. Biodegradation 6:83–92 [CrossRef]
    [Google Scholar]
  19. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  20. Scholz H. C., Tomaso H., Al Dahouk S., Witte A., Schloter M., Kämpfer P., Falsen E., Neubauer H. 2006; Genotyping of Ochrobacterium anthropi by recA -based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis. FEMS Microbiol Lett 257:7–16 [CrossRef]
    [Google Scholar]
  21. Teyssier C., Marchandin H., Jean-Pierre H., Masnou A., Dusart G., Jumas-Bilak E. 2007; Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae , isolated from man. Int J Syst Evol Microbiol 57:1007–1013 [CrossRef]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  23. Tindall B. J. 1990; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
  24. Tripathi A. K., Verma S. C., Chowdhury S. P., Lebuhn M., Gattinger A., Schloter M. 2006; Ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in India. Int J Syst Evol Microbiol 56:1677–1680 [CrossRef]
    [Google Scholar]
  25. Trujillo M. E., Willems A., Abril A., Planchuelo A. M., Rivas R., Ludeña D., Mateos P. F., Martínez-Molina E., Velázquez E. 2005; Nodulation of Lupinus by strains of the new species Ochrobactrum lupini sp. nov. Appl Environ Microbiol 71:1318–1327 [CrossRef]
    [Google Scholar]
  26. Velasco J., Romero C., López-Goñi I., Leiva J., Díaz R., Moriyón I. 1998; Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp. Int J Syst Bacteriol 48:759–768 [CrossRef]
    [Google Scholar]
  27. Ventosa A., Marquez M. C., Kocur M., Tindall B. J. 1993; Comparative study of “ Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . Int J Syst Bacteriol 43:245–248 [CrossRef]
    [Google Scholar]
  28. Yokota A., Akagawa-Matsushita M., Hiraishi A., Katayama Y., Urakami T., Yamasato K. 1992; Distribution of quinone systems in microorganisms: Gram-negative Eubacteria. Bull Jpn Fed Cult Coll 8:136–171
    [Google Scholar]
  29. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
  30. Zurdo-Piñeiro J. L., Rivas R., Trujillo M. E., Vizcaíno N., Carrasco J. A., Chamber M., Palomares A., Mateos P. F., Martínez-Molina E., Velázquez E. 2007; Ochrobactrum cytisi sp. nov. isolated from nodules of Cytisus scoparius in Spain. Int J Syst Evol Microbiol 57:784–788 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65066-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65066-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error