1887

Abstract

An actinomycete, designated strain E71, was isolated from the stem of Wall, a Chinese medicinal plant, and subjected to a polyphasic taxonomic study. Phylogenetic analyses based on the 16S rRNA gene sequence showed that the organism was a member of the genus , and formed a distinct phyletic line distantly related to recognized species of the genus . Morphological and chemotaxonomic data supported the affiliation of strain E71 to the genus . A number of physiological properties and a unique menaquinone profile allowed differentiation of the strain from related species. It is therefore proposed that strain E71 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is E71 (=CGMCC 4.3147=DSM 45047).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65064-0
2007-09-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/9/1995.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65064-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Evol Microbiol 45, 240–245.
    [Google Scholar]
  3. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  4. Coombs, J. T. & Franco, C. M. M. ( 2003; ). Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol 69, 5603–5608.[CrossRef]
    [Google Scholar]
  5. Cote, R., Daggett, P.-M., Gantt, M. J., Hay, R., Jong, S.-C. & Pienta, P. ( 1984; ). ATCC Media Handbook. Rockville, MD: American Type Culture Collection.
  6. Evtushenko, L. I., Taptykova, S. D., Akimov, V. N., Semyonova, S. A. & Kalakoutskii, L. V. ( 1991; ). Glycomyces tenuis sp. nov. Int J Syst Bacteriol 41, 154–157.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  10. Gu, Q., Luo, H., Zheng, W., Liu, Z. & Huang, Y. ( 2006; ). Pseudonocardia oroxyli sp. nov., a novel actinomycete isolated from the surface-sterilized Oroxylum indicum root. Int J Syst Evol Microbiol 56, 2193–2197.[CrossRef]
    [Google Scholar]
  11. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  12. Jones, K. L. ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57, 141–145.
    [Google Scholar]
  13. Kämpfer, M. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  14. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  15. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  16. Labeda, D. P. & Kroppenstedt, R. M. ( 2004; ). Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol 54, 2343–2346.[CrossRef]
    [Google Scholar]
  17. Labeda, D. P. & Kroppenstedt, R. M. ( 2005; ). Stackebrandtia nassauensis gen. nov., sp. nov. and emended description of the family Glycomycetaceae. Int J Syst Evol Microbiol 55, 1687–1691.[CrossRef]
    [Google Scholar]
  18. Labeda, D. P., Testa, R. T., Lechevalier, M. P. & Lechevalier, H. A. ( 1985; ). Glycomyces, a new genus of the Actinomycetales. Int J Syst Bacteriol 35, 417–421.[CrossRef]
    [Google Scholar]
  19. Lechevalier, H. A. & Lechevalier, M. P. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, Special Publication no. 6, pp. 277–284. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society of Industrial Microbiology.
  20. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  21. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  22. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  23. Sasser, M. ( 1990; ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newwark, DE: MIDI Inc.
  24. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  25. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  26. Uchida, K., Kudo, T., Suzuki, K. & Nakase, T. ( 1999; ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45, 49–56.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65064-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65064-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 1995 - 1998

Fatty acid content (%) of strain E71 and related species

Menaquinone profiles of strain E71 and related species

Scanning electron micrograph of strain E71 [Single PDF file](239 KB)

 



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error