Four Gram-positive, catalase-negative, coccoid-shaped isolates were obtained from the caecum and rectum of horses with oligofructose-induced equine laminitis. Phenotypic and phylogenetic studies were performed on these isolates. Initial biochemical profiling assigned two of the isolates to . The other two isolates, however, could not be assigned conclusively on the basis of their biochemical profiles. Gene sequence analysis demonstrated that the four new isolates were related most closely to based on the 16S rRNA gene and to based on the manganese-dependent superoxide dismutase gene (). Sequence divergence values from recognized species based on these two genes were >3 and >13 %, respectively, for all four isolates. Phylogenetic and phenotypic analyses demonstrated that the four isolates formed two distinct clonal groups that are suggested to represent two novel species of the genus . The names proposed for these organisms are sp. nov. (type strain 126 =ATCC BAA-1484 =DSM 19005) and sp. nov. (type strain 151 =ATCC BAA-1485 =DSM 19004).


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vol. , part 1, pp. 262 - 266

Extended phylogenetic tree showing the positions of strains 126 and 151 and their closest relatives based on 16S rRNA gene sequences. All four of the new isolates were fully sequenced; however, only one representative of each of the novel species described is illustrated in this phylogenetic tree as the two isolates for each species had identical 16S rRNA gene sequences. Bar, 10 substitutions per 100 nucleotides.


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