1887

Abstract

A Gram-negative, non-motile, chemoheterotrophic, facultatively aerobic, short-rod-shaped bacterium, designated IMCC1097, was isolated from coastal seawater (10 m depth) of the East Sea, Korea. The temperature, pH and NaCl ranges for growth were 15–30 °C, pH 5.0–10.0 and 1.5–10 % NaCl. The colonies of the strain were very small, having a mean diameter of 0.05 mm. 16S rRNA gene sequence data indicated that the strain was most closely related to genera within the class . Members of the most closely related genera showed less than 90 % sequence similarity and included (89.3 %), (88.7 %), (88.2 %), (86.4–87.6 %) and (87.6 %), which represent five different families of the order . Phylogenetic analyses showed that this marine strain represented a distinct phylogenetic lineage in the order and could not be assigned to any of the defined families in the order. The predominant fatty acids were C 7 and/or iso-C 2-OH, C 7 and C 3-OH, and the DNA G+C content was 57.9 mol%. These chemotaxonomic properties, together with phenotypic characteristics, served to differentiate the strain from phylogenetically closely related genera. The very low sequence similarities (<90 %) and distant relationships between IMCC1097 and members of the order suggested that the strain merited classification within a novel genus within a novel family in the order. On the basis of taxonomic evidence collected in this study, a novel genus and species are proposed, gen. nov., sp. nov., within a new family fam. nov. Strain IMCC1097 (=KCCM 42360 =NBRC 102074) is the type strain of .

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2007-08-01
2024-03-29
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References

  1. Baik K. S., Seong C. N., Kim E. M., Yi H., Bae K. S., Chun J. 2005; Hahella ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 55:681–684 [CrossRef]
    [Google Scholar]
  2. Bruns A., Berthe-Corti L. 1999 Fundibacter jadensis gen. nov., sp. nov., a new slightly halophilic bacterium, isolated from intertidal sediment. Int J Syst Bacteriol 49441–448 [CrossRef]
  3. Cho J.-C., Giovannoni S. J. 2003; Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α - Proteobacteria . Int J Syst Evol Microbiol 53:1031–1036 [CrossRef]
    [Google Scholar]
  4. Cho J. C., Giovannoni S. J. 2006; Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 56:855–859 [CrossRef]
    [Google Scholar]
  5. Fernández-Martínez J., Pujalte M. J., García-Martínez J., Mata M., Garay E., Rodríguez-Valeral F. 2003; Description of Alcanivorax venustensis sp. nov. and reclassification of Fundibacter jadensis DSM 12178T (Bruns and Berthe-Corti 1999) as Alcanivorax jadensis comb. nov., members of the emended genus Alcanivorax . Int J Syst Evol Microbiol 53:331–338 [CrossRef]
    [Google Scholar]
  6. Franzmann P. D., Wehmeyer U., Stackebrandt E. 1988; Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya . Syst Appl Microbiol 11:16–19 [CrossRef]
    [Google Scholar]
  7. Garrity G. M., Bell J. A., Lilburn T. 2005a; Order VIII. Oceanospirillales ord. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2 part B Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. p– 270 New York: Springer;
    [Google Scholar]
  8. Garrity G. M., Bell J. A., Lilburn T. 2005b; Family I. Oceanospirillaceae fam. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2 part B Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. p– 271 New York: Springer;
    [Google Scholar]
  9. Garrity G. M., Bell J. A., Lilburn T. 2005c; Family III. Hahellaceae fam. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2 part B Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. p– 299 New York: Springer;
    [Google Scholar]
  10. Golyshin P. N., Chernikova T. N., Abraham W. R., Lunsdorf H., Timmis K. N., Yakimov M. M. 2002; Oleiphilaceae fam. nov., to include Oleiphilus messinensis gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons. Int J Syst Evol Microbiol 52:901–911 [CrossRef]
    [Google Scholar]
  11. Golyshin P. N., Harayama S., Timmis K. N., Yakimov M. M. 2005; Family II. Alcanivoraceae fam. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2 part B Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. p– 295 New York: Springer;
    [Google Scholar]
  12. Kovács N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703
    [Google Scholar]
  13. Labrenz M., Lawson P. A., Tindall B. J., Collins M. D., Hirsch P. 2003; Saccharospirillum impatiens gen. nov., sp. nov., a novel γ - Proteobacterium isolated from hypersaline Ekho Lake (East Antarctica). Int J Syst Evol Microbiol 53:653–660 [CrossRef]
    [Google Scholar]
  14. Lee H. K., Chun J., Moon E. Y., Ko S. H., Lee D. S., Lee H. S., Bae K. S. 2001; Hahella chejuensis gen. nov., sp. nov. an extracellular-polysaccharide-producing marine bacterium. Int J Syst Evol Microbiol 51661–666
    [Google Scholar]
  15. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [CrossRef]
    [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  18. Ostle A. G., Holt J. G. 1982; Nile blue A as a fluorescent stain for poly- β -hydroxybutyrate. Appl Environ Microbiol 44:238–241
    [Google Scholar]
  19. Prabagaran S. R., Manorama R., Delille D., Shivaji S. 2007; Predominance of Roseobacter , Sulfitobacter , Glaciecola and Psychrobacter in seawater collected off Ushuaia, Argentina, Sub-Antarctica. FEMS Microbiol Ecol 59:342–355
    [Google Scholar]
  20. Romanenko L. A., Schumann P., Rohde M., Mikhailov V. V., Stackebrandt E. 2004; Reinekea marinisedimentorum gen. nov., sp. nov. a novel gammaproteobacterium from marine coastal sediments. Int J Syst Evol Microbiol 54:669–673 [CrossRef]
    [Google Scholar]
  21. Schäfer H., Servais P., Muyzer G. 2000; Successional changes in the genetic diversity of a marine bacterial assemblage during confinement. Arch Microbiol 173:138–145 [CrossRef]
    [Google Scholar]
  22. Swofford D. 2002 paup*: phylogenetic analysis using parsimony (* and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  23. Yakimov M. M., Golyshin P. N., Lang S., Moore E. R., Abraham W. R., Lunsdorf H., Timmis K. N. 1998; Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48:339–348 [CrossRef]
    [Google Scholar]
  24. Yi H., Chang Y. H., Oh H. W., Bae K. S., Chun J. 2003; Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments. Int J Syst Evol Microbiol 53:1013–1018 [CrossRef]
    [Google Scholar]
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