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Abstract

A Gram-negative, non-motile, chemoheterotrophic, facultatively aerobic, short-rod-shaped bacterium, designated IMCC1097, was isolated from coastal seawater (10 m depth) of the East Sea, Korea. The temperature, pH and NaCl ranges for growth were 15–30 °C, pH 5.0–10.0 and 1.5–10 % NaCl. The colonies of the strain were very small, having a mean diameter of 0.05 mm. 16S rRNA gene sequence data indicated that the strain was most closely related to genera within the class . Members of the most closely related genera showed less than 90 % sequence similarity and included (89.3 %), (88.7 %), (88.2 %), (86.4–87.6 %) and (87.6 %), which represent five different families of the order . Phylogenetic analyses showed that this marine strain represented a distinct phylogenetic lineage in the order and could not be assigned to any of the defined families in the order. The predominant fatty acids were C 7 and/or iso-C 2-OH, C 7 and C 3-OH, and the DNA G+C content was 57.9 mol%. These chemotaxonomic properties, together with phenotypic characteristics, served to differentiate the strain from phylogenetically closely related genera. The very low sequence similarities (<90 %) and distant relationships between IMCC1097 and members of the order suggested that the strain merited classification within a novel genus within a novel family in the order. On the basis of taxonomic evidence collected in this study, a novel genus and species are proposed, gen. nov., sp. nov., within a new family fam. nov. Strain IMCC1097 (=KCCM 42360 =NBRC 102074) is the type strain of .

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2007-08-01
2020-01-25
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vol. , part 8, pp. 1793 - 1798

Transmission electron micrographs of negatively stained cells of strain IMCC1097 showing the Gram-negative-type intracellular membranes (a) and a cell dividing by binary fission (b). Bars, 0.2 µm.



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