1887

Abstract

Two novel bacterial strains were isolated from sandy soil from Cox's Bazar, Bangladesh. Strains 5-10-10 and 5-38-42 were Gram-positive, aerobic, non-motile actinomycetes that form branched substrate and aerial mycelium. On the basis of 16S rRNA gene sequence similarity studies, the novel strains were shown to belong to the genus , being most closely related to . Chemotaxonomic data supported the assignment of the novel strains as members of the genus . Strain 5-10-10 contained MK-9(H) and strain 5-38-42 contained MK-9(H) and MK-9(H) as the major menaquinones. Major polar lipids were phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The major cellular fatty acid for strain 5-10-10 was iso-C (26.4 %); C (17.4 %) was the major cellular fatty acid for strain 5-38-42. The results of DNA–DNA hybridization and physiological tests enabled strains 5-10-10 and 5-38-42 to be differentiated genotypically and phenotypically from each other and from the closely related species, . On the basis of these results, strains 5-10-10 and 5-38-42 represent two novel species of the genus . Following an evaluation of morphological, physiological and chemotaxonomic characteristics, 16S rRNA gene sequence comparisons and DNA–DNA hybridization experiments, the new isolates are proposed as two novel species, sp. nov. [type strain, 5-10-10 (=MTCC 8089=JCM 13930)] and sp. nov. [type strain, 5-38-42 (=MTCC 8090=JCM 13931)].

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2007-07-01
2019-10-18
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Supplements

vol. , part 7, pp. 1504 - 1509

Cultural characteristics of strains 5-10-10 and 5-38-42 .

Physiological characteristics of strains 5-10-10 and 5-38-42 .

Chemotaxonomic characteristics and DNA G+C content of strains 5-10-10 and 5-38-42 .

Cellular fatty acid contents (% of total) for strains 5-10-10 and 5-38-42 .

16S rRNA gene sequence similarities (%) between strains 5-10-10 and 5-38-42 and .

DNA-DNA relatedness between strains 5-10-10 and 5-38-42 and JCM 3321 .

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