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Volume 57, Issue 7

sp. nov. and sp. nov. Free

Abstract

Two novel bacterial strains were isolated from sandy soil from Cox's Bazar, Bangladesh. Strains 5-10-10 and 5-38-42 were Gram-positive, aerobic, non-motile actinomycetes that form branched substrate and aerial mycelium. On the basis of 16S rRNA gene sequence similarity studies, the novel strains were shown to belong to the genus , being most closely related to . Chemotaxonomic data supported the assignment of the novel strains as members of the genus . Strain 5-10-10 contained MK-9(H) and strain 5-38-42 contained MK-9(H) and MK-9(H) as the major menaquinones. Major polar lipids were phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The major cellular fatty acid for strain 5-10-10 was iso-C (26.4 %); C (17.4 %) was the major cellular fatty acid for strain 5-38-42. The results of DNA–DNA hybridization and physiological tests enabled strains 5-10-10 and 5-38-42 to be differentiated genotypically and phenotypically from each other and from the closely related species, . On the basis of these results, strains 5-10-10 and 5-38-42 represent two novel species of the genus . Following an evaluation of morphological, physiological and chemotaxonomic characteristics, 16S rRNA gene sequence comparisons and DNA–DNA hybridization experiments, the new isolates are proposed as two novel species, sp. nov. [type strain, 5-10-10 (=MTCC 8089=JCM 13930)] and sp. nov. [type strain, 5-38-42 (=MTCC 8090=JCM 13931)].

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Keyword(s): DAP, diaminopimelic acid , ISP, International Streptomyces Project , OH-PE, hydroxyphosphatidylethanolamine and PE, phosphatidylethanolamine
SGM
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2007-07-01
2024-04-20
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Nonomuraea bangladeshensis sp. nov. and Nonomuraea coxensis sp. nov.
Int J Syst Evol Microbiol 57, 1504 (2007); https://doi.org/10.1099/ijs.0.65054-0
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