%0 Journal Article %A Cheng, Lei %A Qiu, Tian-Lei %A Yin, Xiao-Bo %A Wu, Xiao-Lei %A Hu, Guo-Quan %A Deng, Yu %A Zhang, Hui %T Methermicoccus shengliensis gen. nov., sp. nov., a thermophilic, methylotrophic methanogen isolated from oil-production water, and proposal of Methermicoccaceae fam. nov. %D 2007 %J International Journal of Systematic and Evolutionary Microbiology, %V 57 %N 12 %P 2964-2969 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.65049-0 %K HS-coM, coenzyme M %I Microbiology Society, %X A thermophilic, methylotrophic methanogen, strain ZC-1T, was isolated from the Shengli oilfield, China. Cells of strain ZC-1T were motile cocci, 0.7–1.0 μm in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1T suggested the presence of a proteinaceous cell wall. Strain ZC-1T used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0–6.5, 65 °C, 0.3–0.5 M NaCl and 0.05–0.20 M MgCl2. The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the mcrA gene of strain ZC-1T indicated that it is related specifically to members of the family Methanosaetaceae (90.6 and 76.6 % sequence similarity, respectively). However, strain ZC-1T failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family Methanosaetaceae. Based on these phenotypic and phylogenic characteristics, strain ZC-1T is proposed to represent a novel genus and species, for which the name Methermicoccus shengliensis gen. nov., sp. nov. is proposed. The type strain is ZC-1T (=CGMCC 1.5056T=DSM 18856T). Methermicoccaceae fam. nov. is also proposed. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65049-0