1887

Abstract

A thermophilic, methylotrophic methanogen, strain ZC-1, was isolated from the Shengli oilfield, China. Cells of strain ZC-1 were motile cocci, 0.7–1.0 μm in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1 suggested the presence of a proteinaceous cell wall. Strain ZC-1 used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0–6.5, 65 °C, 0.3–0.5 M NaCl and 0.05–0.20 M MgCl. The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the gene of strain ZC-1 indicated that it is related specifically to members of the family (90.6 and 76.6 % sequence similarity, respectively). However, strain ZC-1 failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family . Based on these phenotypic and phylogenic characteristics, strain ZC-1 is proposed to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is ZC-1 (=CGMCC 1.5056=DSM 18856). fam. nov. is also proposed.

Keyword(s): HS-coM, coenzyme M
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65049-0
2007-12-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/12/2964.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65049-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R. & Wolfe, R. S. ( 1979; ). Methanogens: reevaluation of a unique biological group. Microbiol Rev 43, 260–296.
    [Google Scholar]
  3. Banning, N., Brock, F., Fry, J. C., Parkes, R. J., Hornibrook, E. R. C. & Weightman, A. J. ( 2005; ). Investigation of the methanogen population structure and activity in a brackish lake sediment. Environ Microbiol 7, 947–960.[CrossRef]
    [Google Scholar]
  4. Biavati, B., Vasta, M. & Ferry, J. G. ( 1988; ). Isolation and characterization of “Methanosphaera cuniculi” sp. nov. Appl Environ Microbiol 54, 768–771.
    [Google Scholar]
  5. Boone, D. R. & Kamagata, Y. ( 1998; ). Rejection of the species Methanothrix soehngenii VP and the genus Methanothrix VPas nomina confusa, and transfer of Methanothrix thermophila VP to the genus Methanosaeta VP as Methanosaeta thermophila comb. nov. Request for an opinion. Int J Syst Bacteriol 48, 1079–1080.[CrossRef]
    [Google Scholar]
  6. Boone, D. R. & Whitman, W. B. ( 1988; ). Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int J Syst Bacteriol 38, 212–219.[CrossRef]
    [Google Scholar]
  7. DSMZ ( 1993; ). Catalogue of Strains 1993. Braunschweig, Germany: Deutsche Sammlung von Mikroorganismen und Zellkulturen.
  8. Garrity, G. M. & Holt, J. G. ( 2001; ). Phylum AII. Euryarchaeota phy. nov. In The Archaea and the Deeply Branching and Phototrophic Bacteria (Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1), pp. 211–294. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer-Verlag.
  9. Hales, B. A., Edwards, C., Ritchie, D. A., Hall, G., Pickup, R. W. & Saunders, J. R. ( 1996; ). Isolation and identification of methanogen-specific DNA from blanket bog peat by PCR amplification and sequence analysis. Appl Environ Microbiol 62, 668–675.
    [Google Scholar]
  10. Hungate, R. E. ( 1969; ). A roll-tube method for cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  11. Jarrell, K. F., Faguy, D., Hebert, A. M. & Kalmokoff, M. L. ( 1992; ). A general method of isolating high molecular weight DNA from methanogenic archaea (archaebacteria). Can J Microbiol 38, 65–68.[CrossRef]
    [Google Scholar]
  12. Jiang, B., Parshina, S. N., van Doesburg, W., Lomans, B. P. & Stams, A. J. M. ( 2005; ). Methanomethylovorans thermophila sp. nov., a thermophilic, methylotrophic methanogen from an anaerobic reactor fed with methanol. Int J Syst Evol Microbiol 55, 2465–2470.[CrossRef]
    [Google Scholar]
  13. Kamagata, Y. & Mikami, E. ( 1991; ). Isolation and characterization of a novel thermophilic Methanosaeta strain. Int J Syst Bacteriol 41, 191–196.[CrossRef]
    [Google Scholar]
  14. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  15. Lai, M. C. & Chen, S. C. ( 2001; ). Methanofollis aquaemaris sp. nov., a methanogen isolated from an aquaculture fish pond. Int J Syst Evol Microbiol 51, 1873–1880.[CrossRef]
    [Google Scholar]
  16. Lomans, B. P., Maas, R., Luderer, R., Op den Camp, H. J. M., Pol, A., van der Drift, C. & Vogels, G. D. ( 1999; ). Isolation and characterization of Methanomethylovorans hollandica gen. nov., sp. nov., isolated from freshwater sediment, a methylotrophic methanogen able to grow on dimethyl sulfide and methanethiol. Appl Environ Microbiol 65, 3641–3650.
    [Google Scholar]
  17. Ma, K., Liu, X. & Dong, X. ( 2006; ). Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor. Int J Syst Evol Microbiol 56, 127–131.[CrossRef]
    [Google Scholar]
  18. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  19. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  20. Mikucki, J. A., Liu, Y., Delwiche, M., Colwell, F. S. & Boone, D. R. ( 2003; ). Isolation of a methanogen from deep marine sediments that contain methane hydrates, and description of Methanoculleus submarinus sp. nov. Appl Environ Microbiol 69, 3311–3316.[CrossRef]
    [Google Scholar]
  21. Ollivier, B., Lombardo, A. & Garcia, J. L. ( 1984; ). Isolation and characterization of a new thermophilic Methanosarcina strain (strain MP). Ann Microbiol (Paris) 135B, 187–198.
    [Google Scholar]
  22. Ollivier, B., Cayol, J. L., Patel, B. K., Magot, M., Fardeau, M. L. & Garcia, J. L. ( 1997; ). Methanoplanus petrolearius sp. nov., a novel methanogenic bacterium from an oil-producing well. FEMS Microbiol Lett 147, 51–56.[CrossRef]
    [Google Scholar]
  23. Patel, G. B. & Sprott, G. D. ( 1990; ). Methanosaeta concilii gen. nov., sp. nov.(“Methanothrix concilii”) and Methanosaeta thermoacetophila nom. rev., comb. nov. Int J Syst Bacteriol 40, 79–82.[CrossRef]
    [Google Scholar]
  24. Powell, G. E. ( 1983; ). Interpreting gas kinetics of batch cultures. Biotechnol Lett 5, 437–440.[CrossRef]
    [Google Scholar]
  25. Sprenger, W. W., van Belzen, M. C., Rosenberg, J., Hackstein, J. H. P. & Keltjens, J. T. ( 2000; ). Methanomicrococcus blatticola gen. nov., sp. nov., a methanol- and methylamine-reducing methanogen from the hindgut of the cockroach Periplaneta americana. Int J Syst Evol Microbiol 50, 1989–1999.[CrossRef]
    [Google Scholar]
  26. Takai, K., Inoue, A. & Horikoshi, K. ( 2002; ). Methanothermococcus okinawensis sp. nov., a thermophilic, methane-producing archaeon isolated from a Western Pacific deep-sea hydrothermal vent system. Int J Syst Evol Microbiol 52, 1089–1095.[CrossRef]
    [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  28. Touzel, J. P., Petroff, D. & Albagnac, G. ( 1985; ). Isolation and characterization of a new thermophilic Methanosarcina, the strain CHTI 55. Syst Appl Microbiol 6, 66–71.[CrossRef]
    [Google Scholar]
  29. Zhang, H. & Zhao, Y. ( 1987; ). Isolation and characteristics of Methanosarcina thermophila CB. Acta Microbiol Sin 27, 195–200 (in Chinese).
    [Google Scholar]
  30. Zhao, Y., Zhang, H., Boone, D. R. & Mah, R. A. ( 1986; ). Isolation and characterization of a fast-growing, thermophilic methanobacterium species. Appl Environ Microbiol 52, 1227–1229.
    [Google Scholar]
  31. Zhilina, T. N. & Zavarzin, G. A. ( 1987; ). Methanohalobium evestigatus, n. gen., n. sp. The extremely halophilic methanogenic Archaebacterium. Dokl Akad Nauk SSSR 293, 464–468 (in Russian).
    [Google Scholar]
  32. Zinder, S. H. & Mah, R. A. ( 1979; ). Isolation and characterization of a thermophilic strain of methanosarcina unable to use H2–CO2 for methanogenesis. Appl Environ Microbiol 38, 996–1008.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65049-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65049-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error