1887

Abstract

A thermophilic, methylotrophic methanogen, strain ZC-1, was isolated from the Shengli oilfield, China. Cells of strain ZC-1 were motile cocci, 0.7–1.0 μm in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1 suggested the presence of a proteinaceous cell wall. Strain ZC-1 used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0–6.5, 65 °C, 0.3–0.5 M NaCl and 0.05–0.20 M MgCl. The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the gene of strain ZC-1 indicated that it is related specifically to members of the family (90.6 and 76.6 % sequence similarity, respectively). However, strain ZC-1 failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family . Based on these phenotypic and phylogenic characteristics, strain ZC-1 is proposed to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is ZC-1 (=CGMCC 1.5056=DSM 18856). fam. nov. is also proposed.

Keyword(s): HS-coM, coenzyme M
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2007-12-01
2020-06-03
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