Two bacterial strains isolated from air samples, 5317S-33 and 5413S-11, were characterized by determining their phenotypic characteristics, cellular fatty acid profiles and phylogenetic positions based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that these isolates belonged to the genus . Strain 5317S-33 was most closely related to AR27 (97.9 % sequence similarity). Strain 5413S-11 was most closely related to DSM 5686 (97.3 % sequence similarity), CBMB20 (97.1 % similarity) and JCM 2831 (97.0 % similarity). Cells of both strains were strictly aerobic, Gram-negative, motile and rod-shaped. The major fatty acid was C 7. The G+C contents of the genomic DNA were 68.0 mol% for strain 5317S-33 and 73.2 mol% for strain 5413S-11. According to DNA–DNA hybridization data, strain 5317S-33 showed a level of DNA–DNA relatedness of 33 % with DSM 17169, and strain 5413S-11 showed low levels of DNA–DNA relatedness (<35 %) with DSM 5686, CBMB20 and DSM 1819. On the basis of this polyphasic analysis, it was concluded that strains 5317S-33 and 5413S-11 represent two novel species within the genus , for which the names sp. nov. (type strain 5317S-33 =KACC 11765 =DSM 19015) and sp. nov. (type strain 5413S-11 =KACC 11766 =DSM 19013) are proposed.


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vol. , part 1, pp. 93 - 96

Cellular fatty acid compositions (%) of strains 5317S-33 and 5413S-11 and closely related species. [PDF](42 KB)


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