1887

Abstract

A Gram-negative, non-motile, rod-shaped bacterial strain, designated CW-E 2, was isolated from a polluted soil sample collected from Jiangsu Province, China. A taxonomic study of the isolate, including phylogenetic analysis based on 16S rRNA gene sequences and phenotypic characteristics, was carried out. The predominant menaquinone was MK-6 and the major fatty acids were i-C, i-C 3-OH, i-C 9 and summed feature 4. The G+C content of the DNA was 37.2 mol%. Based on phenotypic and genotypic characteristics, strain CW-E 2 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is CW-E 2 (=KCTC 12877=CCTCC AB 206147).

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2007-08-01
2020-12-02
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References

  1. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  3. Bernardet J.-F., Hugo C., Bruun B. 2006; The genera Chryseobacterium and Elizabethkingia . In The Prokaryotes : Handbook on the Biology of Bacteria . , 3rd edn. vol 7 pp 638–676 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer-Verlag;
  4. de Beer H., Hugo C.-J., Jooste P.-J., Willems A., Vancanneyt M., Coenye T., Vandamme P. 2005; Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant. Int J Syst Evol Microbiol 55:2149–2153 [CrossRef]
    [Google Scholar]
  5. de Beer H., Hugo C.-J., Jooste P.-J., Vancanneyt M., Coenye T., Vandamme P. 2006; Chryseobacterium piscium sp. nov., isolated from fish of the South Atlantic Ocean off South Africa. Int J Syst Evol Microbiol 56:1317–1322 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  9. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Hu H.-Y., Lim B.-R., Naohiro G., Koich F.-J. 2001; Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 47:17–24 [CrossRef]
    [Google Scholar]
  11. Hugo C.-J., Segers P., Hoste B., Vancanneyt M., Kersters K. 2003; Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53:771–777 [CrossRef]
    [Google Scholar]
  12. Kim M. K., Im W. T., Shin Y. K., Lim J. H., Kim S. H., Lee B. C., Park M. Y., Lee K. Y., Lee S. T. 2004; Kaistella koreensis gen. nov., sp. nov. a novel member of the Chryseobacterium–Bergeyella–Riemerella branch. Int J Syst Evol Microbiol 542319–2324 [CrossRef]
    [Google Scholar]
  13. Kim K. K., Bae H.-S., Schumann P., Lee S. T. 2005a; Chryseobacterium daecheongense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 55:133–138 [CrossRef]
    [Google Scholar]
  14. Kim K. K., Kim M. K., Lim J. H., Park H. Y., Lee S. T. 2005b; Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55:1287–1293 [CrossRef]
    [Google Scholar]
  15. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  16. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  17. Kumar S., Tamura K., Jakobsen I.-B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  18. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  19. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  20. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Reichenbachia agariperforans gen. nov., sp. nov. a novel marine bacterium in the phylum Cytophaga–Flavobacterium–Bacteroides . Int J Syst Evol Microbiol 53:81–85 [CrossRef]
    [Google Scholar]
  21. Nedashkovskaya O. I., Kim S. B., Suzuki M., Shevchenko L. S., Lee M. S., Lee K. H., Park M. S., Frolova G. M., Oh H. W. other authors 2005; Pontibacter actiniarum gen. nov., sp. nov. a novel member of the phylum ‘ Bacteroidetes ’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al . 2003. Int J Syst Evol Microbiol 55:2583–2588 [CrossRef]
    [Google Scholar]
  22. Quan Z.-X., Kim K. K., Kim M. K., Jin L., Lee S. T. 2007; Chryseobacterium caeni sp. nov., isolated from bioreactor sludge. Int J Syst Evol Microbiol 57:141–145 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Sambrook J., Russell D. W. 2002; Molecular Cloning: a Laboratory Manual . pp 1595–1596, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  25. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsletter 20:1–6
    [Google Scholar]
  26. Shimomura K., Kaji S., Hiraishi A. 2005; Chryseobacterium shigense sp. nov., a yellow-pigmented, aerobic bacterium isolated from a lactic acid beverage. Int J Syst Evol Microbiol 55:1903–1906 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4888 [CrossRef]
    [Google Scholar]
  28. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B. 1994 New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen.nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44827–831 [CrossRef]
  29. Xu P., Li W.-J., Xu L.-H., Jiang C.-L. 2003; A microwave-based method for genomic DNA extraction from Actinomycetes. Microbiology 30:82–84 (in Chinese)
    [Google Scholar]
  30. Yamaguchi S., Yokoe M. 2000; A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66:3337–3343 [CrossRef]
    [Google Scholar]
  31. Young C.-C., Kämpfer P., Shen F.-T., Lai W.-A., Arun A. B. 2005; Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426 [CrossRef]
    [Google Scholar]
  32. Zhou Y., Wang X., Liu H., Zhang K.-Y., Zhang Y.-Q., Lai R., Li W.-J. 2007; Pontibacter akesuensis sp. nov., isolated from a desert soil in China. Int J Syst Evol Microbiol 57:321–325 [CrossRef]
    [Google Scholar]
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