1887

Abstract

Three Gram-negative, rod-shaped, non-spore-forming, nitrate-reducing isolates (R-32746, R-32768 and R-32729) were obtained from soil. Analysis of repetitive sequence-based PCR showed that the three isolates represented two different strains. 16S rRNA gene sequence analysis and DNA–DNA hybridization placed them within the genus and revealed that they were genotypically different from each other and from all recognized species. Analysis of the fatty acid composition and physiological and biochemical tests allowed differentiation from their closest phylogenetic neighbours. They are therefore considered to represent two novel species, for which the names sp. nov. and sp. nov. are proposed, with strains R-32768 (=LMG 23958=DSM 18941) and R-32729 (=LMG 23959=DSM 18929), respectively, as the type strains.

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2007-09-01
2024-04-23
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