1887

Abstract

The occurrence of genes encoding nitrogenase and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) was investigated in the members of the family . This family forms a separate phylogenetic lineage within the according to 16S rRNA gene sequence analysis and mostly includes photo- and chemoautotrophic halophilic and haloalkaliphilic bacteria. The gene encoding the large subunit of ‘green-like’ form I RubisCO was found in all strains, except the type strains of and . The gene encoding nitrogenase reductase was present in all investigated species of the phototrophic genera , and , but not of the genus . Unexpectedly, fragments were also obtained for the chemotrophic species and , for which diazotrophic potential has not previously been assumed. The -, - and 16S rRNA gene-based trees were not highly congruent in their branching patterns since, in the ‘RubisCO’ and ‘nitrogenase’ trees, representatives of the are divided in a number of broadly distributed clusters and branches. However, the data obtained may be regarded as evidence of the monophyletic origin of the and genes in most species within the family and mainly corresponded to the current taxonomic structure of this family. The phylogeny of the chemolithoautotrophic sulfur-oxidizers and and the nitrifier deviated significantly from the 16S-rRNA gene-based phylogeny. These species clustered with one of the duplicated genes of the purple sulfur bacterium , a member of the family .

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2007-10-01
2020-01-24
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