1887

Abstract

A Gram-positive, strictly aerobic, motile, rod- or coccoid-shaped bacterium, strain 5516J-36, was isolated from an air sample from Jeju region, Korea, and its taxonomic position was investigated by a polyphasic approach. The organism grew optimally at 30 °C and pH 7.0–8.0. Comparative 16S rRNA gene sequencing studies demonstrated that this strain was highly related phylogenetically to PPLB and DSM 20308, showing 98.9 % sequence similarity to both strains. However, the DNA–DNA reassociation values between 5516J-36 and the type strains of and were low (51 and 48 %, respectively). The peptidoglycan type was A3, the predominant menaquinone was MK-8(H), the polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and an unidentified phosphoglycolipid and the whole-cell sugars were glucose, ribose, rhamnose, xylose and galactose. Mycolic acids were absent. The major fatty acids (>5 % of total fatty acids) were iso-C, iso-C, iso-C, iso-C and anteiso-C. The DNA G+C content was 71.7 mol%. On the basis of the above data, it is proposed that strain 5516J-36 represents a novel species, sp. nov. The type strain of is 5516J-36 (=KACC 20556 =DSM 18562).

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2007-09-01
2020-01-25
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