A Gram-negative, motile, aerobic and oval- or rod-shaped bacterial strain, DS-56T, which is phylogenetically closely related to the genus Devosia, was isolated from soil from Dokdo, Korea. Strain DS-56T grew optimally at pH 6.5–7.5 and 25 °C in the presence of 0.5 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DS-56T joins the cluster comprising Devosia species, at a bootstrap resampling value of 100 %. The levels of 16S rRNA gene sequence similarity between strain DS-56T and the type strains of recognized Devosia species ranged from 95.4 to 96.4 %. Strain DS-56T contained Q-11 as the predominant ubiquinone, unlike Devosia species, which contain Q-10 as the predominant ubiquinone. The novel strain also contained some fatty acids, including branched and hydroxy fatty acids, that are not detected in Devosia species. The DNA G+C content of strain DS-56T was 66.2 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain DS-56T represents a novel species of the genus Devosia, for which the name Devosia insulae sp. nov. is proposed. The type strain is DS-56T (=KCTC 12821T=DSM 17955T).
LeeK.-B.,
LiuC.-T.,
AnzaiY.,
KimH.,
AonoT.,
OyaizuH.2005; The hierarchical system of the ‘ Alphaproteobacteria ’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55:1907–1919[CrossRef]
NakagawaY.,
SakaneT.,
YokotaA.1996 Transfer of “ Pseudomonas riboflavina ” (Foster 1944), a gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev. Int J Syst Bacteriol 4616–22[CrossRef]
RivasR.,
WillemsA.,
Subba-RaoN. S.,
MateosP. F.,
DazzoF. B.,
KroppenstedtR. M.,
Martínez-MolinaE.,
GillisM.,
VelázquezE.2003; Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans , an aquatic legume from India. Syst Appl Microbiol 26:47–53[CrossRef]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849[CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]
VanparysB.,
HeylenK.,
LebbeL.,
De VosP.2005; Devosia limi sp. nov., isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55:1997–2000[CrossRef]
YoonJ.-H.,
KimH.,
KimS.-B.,
KimH.-J.,
KimW. Y.,
LeeS. T.,
GoodfellowM.,
ParkY.-H.1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505[CrossRef]
YoonJ.-H.,
LeeS. T.,
ParkY.-H.1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194[CrossRef]
YoonJ.-H.,
KangK. H.,
ParkY.-H.2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454[CrossRef]
YoonJ.-H.,
KangS.-J.,
LeeS.-Y.,
LeeM.-H.,
OhT.-K.2005a; Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea. Int J Syst Evol Microbiol 55:1833–1837[CrossRef]