A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1, was rod-shaped and motile. The temperature range for growth was 33–51 °C (optimum 44–46 °C) and the pH range was 6.0–7.5 (optimum pH 6.4–7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained -diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C, iso-C 10 and iso-C. Strain RE35E1 was related to representatives of the genera and , the closest relatives being DSM 17038 (96.3 % 16S rRNA gene sequence similarity) and DSM 5475 (92.0 % similarity). Strain RE35E1 represents a novel species, for which the name sp. nov. is proposed. The type strain is RE35E1 (=DSM 16058=JCM 14019).


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