1887

Abstract

Three strains of Gram-negative, aerobic, neutrophilic and halophilic bacteria were isolated from samples of a salt lake on the Qinghai–Tibet Plateau and a subterranean saline well in the Si-Chuan Basin of China. These isolates, designated AJ275, AJ282 and ZG16, were investigated using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the isolates could be affiliated to the genus . Genomic DNA G+C contents were 65.9 mol% for AJ275, 56.7 mol% for AJ282 and 57.6 mol% for ZG16. The results of DNA–DNA hybridizations, fatty acid analysis and physiological and biochemical tests allowed the isolates to be differentiated genotypically and phenotypically from closely related species. It is proposed that strains AJ275 (=CGMCC 1.6493=JCM 14606=LMG 23976), AJ282 (=CGMCC 1.6494=JCM 14607=LMG 23978) and ZG16 (=CGMCC 1.6495=JCM 14608=LMG 23977) represent the type strains of three novel species in the genus : sp. nov., sp. nov. and sp. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65022-0
2007-07-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1619.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65022-0&mimeType=html&fmt=ahah

References

  1. Berendes, F., Gottschalk, G., Heine-Dobbernack, E., Moore, E. R. B. & Tindall, B. J. ( 1996; ). Halomonas desiderata sp. nov., a new alkaliphilic, halotolerant and denitrifying bacterium isolated from a municipal sewage works. Syst Appl Microbiol 19, 158–167.[CrossRef]
    [Google Scholar]
  2. Bouchotroch, S., Quesada, E., Moral, A. D., Llamas, I. & Béjar, V. ( 2001; ). Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 51, 1625–1632.[CrossRef]
    [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Dobson, S. J. & Franzmann, P. D. ( 1996; ). Unification of the genera Deleya (Baumann et al. 1983), Halomonas ( Vreeland et al. 1980 ), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int J Syst Bacteriol 46, 550–558.[CrossRef]
    [Google Scholar]
  5. Duckworth, A. W., Grant, W. D., Jones, B. E., Meijer, D., Márquez, M. C. & Ventosa, A. ( 2000; ). Halomonas magadii sp. nov., a new member of the genus Halomonas, isolated from a soda lake of the East African Rift Valley. Extremophiles 4, 53–60.
    [Google Scholar]
  6. Franzmann, P. D., Burton, H. R. & McMeekin, T. A. ( 1987; ). Halomonas subglaciescola, a new species of halotolerant bacteria isolated from Antarctica. Int J Syst Bacteriol 37, 27–34.[CrossRef]
    [Google Scholar]
  7. Franzmann, P. D., Wehmeyer, U. & Stackebrandt, E. ( 1988; ). Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya. Syst Appl Microbiol 11, 16–19.[CrossRef]
    [Google Scholar]
  8. García, M. T., Mellado, E., Ostos, J. C. & Ventosa, A. ( 2004; ). Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. Int J Syst Evol Microbiol 54, 1723–1728.[CrossRef]
    [Google Scholar]
  9. Heyrman, J., Balcaen, A., De Vos, P. & Swings, J. ( 2002; ). Halomonas muralis sp. nov., isolated from microbial biofilms colonizing the walls and murals of the Saint-Catherine chapel (Castle Herberstein, Austria). Int J Syst Evol Microbiol 52, 2049–2054.[CrossRef]
    [Google Scholar]
  10. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  11. James, S. R., Dobson, S. J., Franzmann, P. D. & McMeekin, T. A. ( 1990; ). Halomonas meridiana, a new species of extremely halotolerant bacteria isolated from Antarctic saline lakes. Syst Appl Microbiol 13, 270–278.[CrossRef]
    [Google Scholar]
  12. Kaye, J. Z., Márquez, M. C., Ventosa, A. & Baross, J. A. ( 2004; ). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int J Syst Evol Microbiol 54, 499–511.[CrossRef]
    [Google Scholar]
  13. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  14. Kuykendall, L. D., Roy, M. A., O'Neill, J. J. & Devine, T. E. ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef]
    [Google Scholar]
  15. Lee, J.-C., Jeon, C.-O., Lim, J.-M., Lee, S.-M., Lee, J.-M., Song, S.-M., Park, D.-J., Li, W.-J. & Kim, C.-J. ( 2005; ). Halomonas taeanensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 55, 2027–2032.[CrossRef]
    [Google Scholar]
  16. Lim, J.-M., Yoon, J.-H., Lee, J.-C., Jeon, C.-O., Park, D.-J., Sung, C. & Kim, C.-J. ( 2004; ). Halomonas koreensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 54, 2037–2042.[CrossRef]
    [Google Scholar]
  17. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  18. Martínez-Cánovas, M. J., Quesada, E., Llamas, I. & Béjar, V. ( 2004a; ). Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 54, 733–737.[CrossRef]
    [Google Scholar]
  19. Martínez-Cánovas, M. J., Béjar, V., Martínez-Checa, F. & Quesada, E. ( 2004b; ). Halomonas anticariensis sp. nov., from Fuente de Piedra, a saline-wetland wildfowl reserve in Málaga, southern Spain. Int J Syst Evol Microbiol 54, 1329–1332.[CrossRef]
    [Google Scholar]
  20. Martínez-Checa, F., Béjar, V., Martínez-Cánovas, M. J., Llamas, I. & Quesada, E. ( 2005; ). Halomonas almeriensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium from Cabo de Gata, Almería, south-east Spain. Int J Syst Evol Microbiol 55, 2007–2011.[CrossRef]
    [Google Scholar]
  21. Mata, J. A., Martinez-Canovas, J., Quesada, E. & Bejar, V. ( 2002; ). A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef]
    [Google Scholar]
  22. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A. ( 1995; ). Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45, 712–716.[CrossRef]
    [Google Scholar]
  23. Mormile, M. R., Romine, M. F., Garcia, M. T., Ventosa, A., Bailey, T. J. & Peyton, B. M. ( 1999; ). Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. Syst Appl Microbiol 22, 551–558.[CrossRef]
    [Google Scholar]
  24. Quillaguamán, J., Hatti-Kaul, R., Mattiasson, B., Alvarez, M. T. & Delgado, O. ( 2004; ). Halomonas boliviensis sp. nov., an alkalitolerant, moderate halophile isolated from soil around a Bolivian hypersaline lake. Int J Syst Evol Microbiol 54, 721–725.[CrossRef]
    [Google Scholar]
  25. Romanenko, L. A., Schumann, P., Rohde, M., Mikhailov, V. V. & Stackebrandt, E. ( 2002; ). Halomonas halocynthiae sp. nov., isolated from the marine ascidian Halocynthia aurantium. Int J Syst Evol Microbiol 52, 1767–1772.[CrossRef]
    [Google Scholar]
  26. Romano, I., Nicolaus, B., Lama, L., Manca, M. C. & Gambacorta, A. ( 1996; ). Characterization of a haloalkalophilic strictly aerobic bacterium, isolated from Pantelleria Island. Syst Appl Microbiol 19, 326–333.[CrossRef]
    [Google Scholar]
  27. Romano, I., Gioradano, A., Lama, L., Nicolaus, B. & Gambacorta, A. ( 2005; ). Halomonas campaniensis sp. nov., a haloalkaliphilic bacterium isolated from a mineral pool of Campania Region, Italy. Syst Appl Microbiol 28, 610–618.[CrossRef]
    [Google Scholar]
  28. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  29. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  30. Vreeland, R. H., Litchfield, C. D., Martin, E. L. & Elliot, E. ( 1980; ). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30, 485–495.[CrossRef]
    [Google Scholar]
  31. Xu, X.-W., Wu, M. & Huang, W.-D. ( 2005; ). Isolation and characterization of a novel strain of Natrinema containing a bop gene. J Zhejiang Univ Sci B 6, 142–146.
    [Google Scholar]
  32. Yoon, J.-H., Choi, S.-H., Lee, K.-C., Kho, Y.-H., Kang, K.-H. & Park, Y.-H. ( 2001; ). Halomonas marisflavae sp. nov., a halophilic bacterium isolated from the Yellow Sea in Korea. Int J Syst Evol Microbiol 51, 1171–1177.[CrossRef]
    [Google Scholar]
  33. Yoon, J.-H., Lee, K.-C., Kho, Y.-H., Kang, K.-H., Kim, C.-J. & Park, Y.-H. ( 2002; ). Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52, 123–130.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65022-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65022-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error