1887

Abstract

A coccoid- to ovoid-shaped, Gram-positive, non-motile bacterial strain, designated phenol-A, was isolated from an oil-contaminated coastal sand sample collected from Pingtung County, southern Taiwan, and characterized by use of a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus in the family , class . The closest neighbours were LMG 19848 (96.9 % 16S rRNA gene sequence similarity), DSM 9573 (97.0 %) and C3H-21 (96.3 %). The peptidoglycan type of strain phenol-A was variation A4 with -diaminopimelic acid as the diagnostic cell-wall diamino acid. The isolate contained MK-7 as the major component of the quinone system. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, and unidentified phospholipids and glycolipids. The predominant fatty acid was anteiso-C (59.5 %); significant amounts of iso-C (9.4 %), iso-C (9.5 %) and anteiso-C (10.8 %) were also present. The isolate was also distinguished from recognized members of the genus on the basis of several phenotypic and biochemical characteristics. It is evident from the genotypic, chemotaxonomic and phenotypic data that isolate phenol-A represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is phenol-A (=LMG 23707=BCRC 17589).

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2007-11-01
2019-10-19
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vol. , part 11, pp. 2674 - 2679

The polar lipid profile of strain phenol-A .

A comparison of the fatty acid profile of strain phenol-A with recognized members of the genus .

[ PDF file of Supplementary Fig. S1 and Supplementary Table Sl.] (93 KB)



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