1887

Abstract

Two bacterial isolates from ginseng fields in Korea, strains GR17-7 and GP18-1, were characterized using a polyphasic approach. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the , and showed that the closest phylogenetic relationships were with members of the genus . The 16S rRNA gene sequence similarity between strains GR17-7 and GP18-1 was 97.2 %. Both strains showed 16S rRNA gene sequence similarities of 95.2–96.9 % to type strains of recognized species. The G+C contents of the DNA of strains GR17-7 and GP18-1 were 61.0 and 62.5 mol%, respectively. According to the DNA–DNA hydridization tests, the hybridization value between strains GR17-7 and GP18-1 was 34 %. Strains GR17-7 and GP18-1 showed less than 32 % DNA–DNA relatedness with KCTC 12098 and LMG 23181. Strains GR17-7 and GP18-1 were aerobic, Gram-negative, rod-shaped, and catalase- and oxidase-positive. Major fatty acids of both strains were iso-C 9 and iso-C. Based on the data presented, two novel species are proposed, with the names sp. nov. (type strain GR17-7=KACC 11762=DSM 18993) and sp. nov. (type strain GP18-1=KACC 11761=DSM 19241).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65018-0
2007-12-01
2021-02-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/12/2810.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65018-0&mimeType=html&fmt=ahah

References

  1. De Clercq D., Van Trappen S., Cleenwerck I., Ceustermans A., Swings J., Coosemans J., Ryckeboer J. 2006; Rhodanobacter spathiphylli sp. nov., a gammaproteobacterium isolated from the roots of Spathiphyllum plants grown in a compost-amended potting mix. Int J Syst Evol Microbiol 56:1755–1759 [CrossRef]
    [Google Scholar]
  2. Hiraishi A. 1992; Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15:210–213 [CrossRef]
    [Google Scholar]
  3. Im W. T., Lee S. T., Yokota A. 2004; Rhodanobacter fulvus sp. nov., a β -galactosidase-producing gammaproteobacterium. J Gen Appl Microbiol 50:143–147 [CrossRef]
    [Google Scholar]
  4. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  5. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  6. Nalin R., Simonet P., Vogel T. M., Normand P. 1999; Rhodanobacter lindaniclasticus gen. nov., sp. nov., a lindane-degrading bacterium. Int J Syst Bacteriol 49:19–23 [CrossRef]
    [Google Scholar]
  7. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans , and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154 [CrossRef]
    [Google Scholar]
  8. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  9. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  10. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65018-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65018-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error