1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 1

gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order , and proposal of fam. nov. Free

Abstract

Two novel Gram-negative bacterial strains, designated CL-GR15 and CL-GR35, were isolated from coastal seawater of the east coast of Korea. Identical 16S rRNA gene sequences were found in the two strains, and it was found that the strains represented a distinct and deep evolutionary lineage of descent in the order , and clustered with yet-uncultured marine bacteria. This lineage could not be associated with any of 12 known families in the order . The most closely related established genus was (90.7–92.5 % sequence similarity) in the family . Cells of the strains were facultatively anaerobic, weakly motile rods which occasionally exhibited cohesion with each other. The strains were catalase-, oxidase- and gelatinase-positive, and accumulated intracellular poly--hydroxybutyrate granules. Ubiquinone 10 was the major quinone. The major polar lipids comprised phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unidentified aminolipid and an unidentified glycolipid. The cellular fatty acids mainly consisted of C 7, summed feature 3 (C iso 2-OH and/or C 7) and C 7. The DNA G+C content was 52.8–53.0 mol%. DNA–DNA hybridization experiments revealed high values (>97 %) for relatedness between strains CL-GR15 and CL-GR35 and suggested that these two strains constituted a single species. The distinct phylogenetic position and combinations of genotypic, phenotypic and chemotaxonomic characteristics support the proposal of gen. nov., with the type species sp. nov. (type strain, CL-GR15=KCCM 42319=DSM 18289). fam. nov. is also proposed.

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2008-01-01
2021-10-15
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Cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales, and proposal of Cohaesibacteraceae fam. nov.
Int J Syst Evol Microbiol 58, 267 (2008); https://doi.org/10.1099/ijs.0.65016-0
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vol. , part 1, pp. 267–277

Neighbour-joining tree showing relationship between the strains studied and most validly published species of related families in the order .

Transmission electron micrograph of negatively stained cells of strain CL-GR15 and strain CL-GR35.

Two-dimensional thin-layer chromatograms of the polar lipids of strain CL-GR15 .

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