Two novel actinomycete strains, designated HFW-18 and HFW-21, were isolated from a water sample around Hwasun Beach on the coast of Jeju Island, Republic of Korea. The cells of the organisms were aerobic, Gram-positive, non-motile rods. The temperature and initial pH ranges for growth were 4–37 °C and pH 5.1–9.1, respectively. They had -diaminopimelic acid in their cell-wall peptidoglycan, MK-8(H) as the major menaquinone and DNA G+C contents of 71.1–72.2 mol%. The cellular fatty acids consisted of straight-chain saturated, branched, monounsaturated and 10-methyl fatty acids, with a major component of iso-C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The isolates were identical in terms of their 16S rRNA gene sequences and BOX-PCR DNA fingerprints. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates occupied distinct positions within the radiation of the genus . According to 16S rRNA gene sequence similarity, the closest relatives were JC2055 (97.4 %), DSM 16090 (97.3 %) and SBS-26 (97.0 %). Levels of 16S rRNA gene sequence similarity between the isolates and other members of the genus were in the range 91.8–95.3 %. On the basis of the phenotypic and molecular genetic data presented here, the isolates represent members of a novel species of the genus . The name sp. nov. is proposed, and the type strain is HFW-21 (=KCTC 19197=DSM 18584).


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  1. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  2. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle.
  4. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  5. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & Schrempf, H.(1985).Genetic Manipulation ofStreptomyces: a Laboratory Manual. Norwich, UK: The John Innes Foundation.
  6. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  7. Kubota, M., Kawahara, K., Sekiya, K., Uchida, T., Hattori, Y., Futamata, H. & Hiraishi, A.(2005).Nocardioides aromaticivorans sp. nov., a dibenzofuran-degrading bacterium isolated from dioxin-polluted environments. Syst Appl Microbiol 28, 165–174.[CrossRef] [Google Scholar]
  8. Lawson, P. A., Collins, M. D., Schumann, P., Tindall, B. J., Hirsch, P. & Labrenz, M.(2000). New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23, 219–229.[CrossRef] [Google Scholar]
  9. Lee, S. D.(2007).Nocardioides furvisabuli sp. nov., isolated from black sand. Int J Syst Evol Microbiol 57, 35–39.[CrossRef] [Google Scholar]
  10. Lee, S. D., Kang, S.-O. & Hah, Y. C.(2000).Hongia gen. nov., a new genus of the order Actinomycetales. Int J Syst Evol Microbiol 50, 191–199.[CrossRef] [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  12. O'Donnell, A. G., Goodfellow, M. & Minnikin, D. E.(1982). Lipids in the classification of Nocardioides: reclassification of Arthrobacter simplex (Jensen) Lochhead in the genus Nocardioides (Prauser) emend. O'Donnell et al. as Nocardioides simplex comb. nov. Arch Microbiol 133, 323–329.[CrossRef] [Google Scholar]
  13. Prauser, H.(1976).Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 58–65.[CrossRef] [Google Scholar]
  14. Rademaker, J. L. W. & de Bruijn, F. J.(1997). Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. In DNA Makers: Protocols, Applications and Overviews, pp. 151–171. Edited by G. Caetano-Anollés & P. M. Gresshoff. New York: Wiley.
  15. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  16. Schippers, A., Schumann, P. & Spröer, C.(2005).Nocardioides oleivorans sp. nov., a novel crude-oil-degrading bacterium. Int J Syst Evol Microbiol 55, 1501–1504.[CrossRef] [Google Scholar]
  17. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  19. Versalovic, J., Schneider, M., de Bruijn, F. J. & Lupski, J. R.(1994). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40. [Google Scholar]
  20. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  21. Yi, H. & Chun, J.(2004).Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 1295–1299.[CrossRef] [Google Scholar]
  22. Yoon, J.-H., Cho, Y. G., Lee, S. T., Suzuki, K., Nakase, T. & Park, Y.-H.(1999).Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium. Int J Syst Bacteriol 49, 675–680.[CrossRef] [Google Scholar]
  23. Yoon, J.-H., Kim, I.-G., Kang, K. H., Oh, T.-K. & Park, Y.-H.(2004).Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. Int J Syst Evol Microbiol 54, 71–75.[CrossRef] [Google Scholar]
  24. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K.(2007).Nocardioides insulae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57, 136–140.[CrossRef] [Google Scholar]

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Cellular fatty acid composition (%) of strains HFW-18 and HFW-21 .


A transmission electron micrograph of a cell of strain HFW-21 grown on trypticase soy broth for 24 h at 30 °C. Bar, 0.2 μm.


Neighbour-joining tree (Saitou & Nei, 1987) showing phylogenetic positions of strains HFW-18 and HFW-21T within the radiation encompassing representatives of the family . Tree construction was based on a total of 1348 nt in unambiguously aligned positions of 16S rRNA gene sequences. KCTC 9080 (M76388) was used as an outgroup. Asterisks at the corresponding branches are those also found in both maximum-likelihood (Felsenstein, 1981) and maximum-parsimony (Fitch, 1971) trees. Numbers at the nodes are percentages of bootstrap support (>50 %), based on a neighbour-joining analysis of 1000 resamplings. Bar, 1 substitution per 100 nt positions.

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