1887

Abstract

Two novel actinomycete strains, designated HFW-18 and HFW-21, were isolated from a water sample around Hwasun Beach on the coast of Jeju Island, Republic of Korea. The cells of the organisms were aerobic, Gram-positive, non-motile rods. The temperature and initial pH ranges for growth were 4–37 °C and pH 5.1–9.1, respectively. They had -diaminopimelic acid in their cell-wall peptidoglycan, MK-8(H) as the major menaquinone and DNA G+C contents of 71.1–72.2 mol%. The cellular fatty acids consisted of straight-chain saturated, branched, monounsaturated and 10-methyl fatty acids, with a major component of iso-C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The isolates were identical in terms of their 16S rRNA gene sequences and BOX-PCR DNA fingerprints. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates occupied distinct positions within the radiation of the genus . According to 16S rRNA gene sequence similarity, the closest relatives were JC2055 (97.4 %), DSM 16090 (97.3 %) and SBS-26 (97.0 %). Levels of 16S rRNA gene sequence similarity between the isolates and other members of the genus were in the range 91.8–95.3 %. On the basis of the phenotypic and molecular genetic data presented here, the isolates represent members of a novel species of the genus . The name sp. nov. is proposed, and the type strain is HFW-21 (=KCTC 19197=DSM 18584).

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2008-01-01
2019-08-19
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Cellular fatty acid composition (%) of strains HFW-18 and HFW-21 .

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A transmission electron micrograph of a cell of strain HFW-21 grown on trypticase soy broth for 24 h at 30 °C. Bar, 0.2 μm.

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Neighbour-joining tree (Saitou & Nei, 1987) showing phylogenetic positions of strains HFW-18 and HFW-21T within the radiation encompassing representatives of the family . Tree construction was based on a total of 1348 nt in unambiguously aligned positions of 16S rRNA gene sequences. KCTC 9080 (M76388) was used as an outgroup. Asterisks at the corresponding branches are those also found in both maximum-likelihood (Felsenstein, 1981) and maximum-parsimony (Fitch, 1971) trees. Numbers at the nodes are percentages of bootstrap support (>50 %), based on a neighbour-joining analysis of 1000 resamplings. Bar, 1 substitution per 100 nt positions.

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