A moderately halophilic, spore-forming, Gram-positive, short-rod-shaped bacterium, designated strain MS10, was isolated from the surface of leaves of the black mangrove and was subjected to a polyphasic taxonomic study. Strain MS10 was able to grow at NaCl concentrations in the range 5–20 % (w/v) with optimum growth at 10 % (w/v) NaCl. Growth occurred at temperatures of 10–50 °C (optimal growth at 33–35 °C) and pH 6.0–9.0 (optimal growth at pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MS10 fell within the branch encompassing members of the genus and was most closely related to JCM 12772 (99.2 % 16S rRNA gene sequence similarity). The DNA G+C content of strain MS10 was 45.7 mol%, the major respiratory isoprenoid quinone was MK-7 and the cell-wall peptidoglycan was of the -Orn–-Asp type, characteristics consistent with its affiliation to the genus . Strain MS10 showed a level of DNA–DNA hybridization with JCM 12772 of 29 % and levels below 70 % were also obtained with respect to other recognized members of the genus . The major fatty acids of strain MS10 were iso-C, anteiso-C, iso-C and iso-C. Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain MS10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MS10 (=CECT 7206=CCM 7397).


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