1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 1

sp. nov., a moderately halophilic bacterium isolated from the black mangrove Free

Abstract

A moderately halophilic, spore-forming, Gram-positive, short-rod-shaped bacterium, designated strain MS10, was isolated from the surface of leaves of the black mangrove and was subjected to a polyphasic taxonomic study. Strain MS10 was able to grow at NaCl concentrations in the range 5–20 % (w/v) with optimum growth at 10 % (w/v) NaCl. Growth occurred at temperatures of 10–50 °C (optimal growth at 33–35 °C) and pH 6.0–9.0 (optimal growth at pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MS10 fell within the branch encompassing members of the genus and was most closely related to JCM 12772 (99.2 % 16S rRNA gene sequence similarity). The DNA G+C content of strain MS10 was 45.7 mol%, the major respiratory isoprenoid quinone was MK-7 and the cell-wall peptidoglycan was of the -Orn–-Asp type, characteristics consistent with its affiliation to the genus . Strain MS10 showed a level of DNA–DNA hybridization with JCM 12772 of 29 % and levels below 70 % were also obtained with respect to other recognized members of the genus . The major fatty acids of strain MS10 were iso-C, anteiso-C, iso-C and iso-C. Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain MS10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MS10 (=CECT 7206=CCM 7397).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65008-0
2008-01-01
2020-04-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/1/125.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65008-0&mimeType=html&fmt=ahah

References

  1. Amoozegar, M. A., Malekzadeh, F., Malik, K. A., Schumann, P. & Sproer, C. ( 2003; ). Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53, 1059–1063.[CrossRef]
    [Google Scholar]
  2. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M. ( 1966; ). Antibiotic susceptibility testing by a standarized single disk method. Am J Clin Pathol 45, 493–496.
     [Google Scholar]
  3. Claus, D., Fahmy, F., Rolf, H. J. & Tosunoglu, N. ( 1983; ). Sporosarcina halophila sp. nov., an obligate, slightly halophilic bacterium from salt marsh soils. Syst Appl Microbiol 4, 496–506.[CrossRef]
    [Google Scholar]
  4. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  5. De Ley, J. & Tijtgat, R. ( 1970; ). Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36, 461–474.[CrossRef]
    [Google Scholar]
  6. Díaz-Muñoz, G. & Montalvo-Rodríguez, R. ( 2005; ). Halophilic black yeast Hortaea werneckii in the Cabo Rojo solar salterns: its first record for this extreme environment in Puerto Rico. Caribb J Sci 41, 360–365.
     [Google Scholar]
  7. Dussault, H. P. ( 1955; ). An improved technique for staining red-halophilic bacteria. J Bacteriol 70, 484–485.
     [Google Scholar]
  8. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Hezayen, F. F., Tindall, B. J., Steinbuchel, A. & Rehm, B. H. A. ( 2002; ). Characterization of a novel halophilic archaeon, Halobiforma haloterrestris gen. nov., sp. nov., and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. Int J Syst Evol Microbiol 52, 2271–2280.[CrossRef]
    [Google Scholar]
  10. Johnson, J. L. ( 1994; ). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–681. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  11. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  12. Koser, S. A. ( 1923; ). Utilization of the salts of organic acids by the colon-aerogenes group. J Bacteriol 8, 493–520.
     [Google Scholar]
  13. Liu, W. Y., Zeng, J., Wang, L., Dou, Y. T. & Yang, S. S. ( 2005; ). Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. Int J Syst Evol Microbiol 55, 1991–1996.[CrossRef]
    [Google Scholar]
  14. Lugo, A. E. & Snedaker, S. C. ( 1975; ). Properties of a mangrove forest in southern Florida. In Actas, International Symposium on Biological Management of Mangroves, pp. 170–212. Edited by G. Walsh, S. C. Snedaker & H. Teas. Gainesville: University of Florida.
  15. Maidak, B. L., Olsen, G. J., Larsen, N., Overbeek, R., McCaughey, M. J. & Woese, C. R. ( 1996; ). The Ribosomal Database Project (RDP). Nucleic Acids Res 24, 82–85.[CrossRef]
    [Google Scholar]
  16. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  17. Miller, L. T. ( 1982; ). Single derivation method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxyl acids. J Clin Microbiol 16, 584–586.
     [Google Scholar]
  18. Montalvo-Rodríguez, R., Vreeland, R. H., Oren, A., Kessel, M., Betancourt, C. & López-Garriga, J. ( 1998; ). Halogeometricum borinquense gen. nov., sp. nov., a novel halophilic archaeon from Puerto Rico. Int J Syst Bacteriol 48, 1305–1312.[CrossRef]
    [Google Scholar]
  19. Montalvo-Rodríguez, R., López-Garriga, J., Vreeland, R. H., Oren, A., Ventosa, A. & Kamekura, M. ( 2000; ). Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico. Int J Syst Evol Microbiol 50, 1065–1071.[CrossRef]
    [Google Scholar]
  20. Mormile, M. R., Romine, M. F., Garcia, M. T., Ventosa, A., Bailey, T. J. & Peyton, B. M. ( 1999; ). Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. Syst Appl Microbiol 22, 551–558.[CrossRef]
    [Google Scholar]
  21. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists (Society for Applied Bacteriology Technical Series no. 14), 2nd edn, pp. 277–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  22. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
     [Google Scholar]
  23. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
     [Google Scholar]
  24. Sehgal, S. N. & Gibbons, N. E. ( 1960; ). Effect of metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6, 165–169.[CrossRef]
    [Google Scholar]
  25. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef]
    [Google Scholar]
  26. Simmons, J. S. ( 1926; ). A culture medium for differentiating organisms of typhoid-colon aerogenes groups and for isolation of certain fungi. J Infect Dis 39, 209–217.[CrossRef]
    [Google Scholar]
  27. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K. H. ( 1996; ). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46, 492–495.[CrossRef]
    [Google Scholar]
  28. Ventosa, A., Quesada, E., Rodríguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
     [Google Scholar]
  29. Ventosa, A., Gutierrez, M. C., Kamekura, M. & Dyall-Smith, M. L. ( 1999; ). Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49, 131–136.[CrossRef]
    [Google Scholar]
  30. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  31. Yoon, J. H., Kang, K. H. & Park, Y. H. ( 2003; ). Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53, 687–693.[CrossRef]
    [Google Scholar]
  32. Yoon, J. H., Kang, K. H., Oh, T. K. & Park, Y. H. ( 2004; ). Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8, 23–28.[CrossRef]
    [Google Scholar]
  33. Yoon, J. H., Kang, S. J., Lee, C. H., Oh, H. W. & Oh, T. K. ( 2005; ). Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 55, 2413–2417.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65008-0
Loading
Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans
Int J Syst Evol Microbiol 58, 125 (2008); https://doi.org/10.1099/ijs.0.65008-0
/content/journal/ijsem/10.1099/ijs.0.65008-0
/content/journal/ijsem/10.1099/ijs.0.65008-0
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error