1887

Abstract

A Gram-positive, non-motile, coccoid-shaped, non-spore-forming halophilic bacterial strain, BY-5, was isolated from a marine solar saltern in Korea and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew optimally at 37 °C and in the presence of 10 % (w/v) NaCl. Strain BY-5 had -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone and anteiso-C, iso-C, anteiso-C and iso-C as the major fatty acids. The DNA G+C content was 47.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BY-5 formed a coherent cluster with and . Strain BY-5 exhibited 16S rRNA gene sequence similarity values of 98.7 and 97.4 % to the type strains of . and . , respectively. Strain BY-5 was distinguished from . and . by several phenotypic properties and DNA–DNA relatedness data. On the basis of the combined chemotaxonomic and phylogenetic data, it is proposed that . . and strain BY-5 should be placed in a new genus as three separate species. and are reclassified in a new genus, gen. nov., as comb. nov. and comb. nov., respectively. The type species of the new genus is . Strain BY-5 (=KCTC 3989=CIP 108918) is placed in the genus as a novel species sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65003-0
2007-10-01
2020-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/10/2406.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65003-0&mimeType=html&fmt=ahah

References

  1. Arahal D. R., Márquez M. C., Volcani B. E., Schleifer K. H., Ventosa A. 1999; Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J Syst Bacteriol 49:521–530 [CrossRef]
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Williams A. M., Wallbanks S. 1990; The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov. FEMS Microbiol Lett 58:255–262
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. García M. T., Gallego V., Ventosa A., Mellado E. 2005; Thalassobacillus devorans gen. nov., sp. nov. a moderately halophilic, phenol-degrading, Gram-positive bacterium. Int J Syst Evol Microbiol 55:1789–1795 [CrossRef]
    [Google Scholar]
  7. Hao M. V., Kocur M., Komagata K. 1984; Marinococcus gen. nov., a new genus for motile cocci with meso -diaminopimelic acid in the cell wall; and Marinococcus albus sp. nov. and Marinococcus halophilus (Novitsky and Kushner) comb. nov. J Gen Appl Microbiol 30:449–459 [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  9. Kocur M., Páčová Z., Hodgkiss W., Martinec T. 1970; The taxonomic status of the genus Planococcus Migula 1894. Int J Syst Bacteriol 20:241–248 [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  11. Kushner D. J., Kamekura M. 1988; Physiology of halophilic eubacteria. In Halophilic Bacteria vol. 1 pp 109–140 Edited by Rodriguez-Valera F. Boca Raton, FL: CRC Press;
    [Google Scholar]
  12. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  14. Li W.-J., Schumann P., Zhang Y.-Q., Chen G.-Z., Tian X.-P., Xu L.-H., Stackebrandt E., Jiang C.-L. 2005; Marinococcus halotolerans sp. nov., isolated from Qinghai, north-west China. Int J Syst Evol Microbiol 55:1801–1804 [CrossRef]
    [Google Scholar]
  15. Li W.-J., Zhang Y.-Q., Schumann P., Tian X.-P., Zhang Y.-Q., Xu L.-H., Jiang C.-L. 2006; Sinococcus qinghaiensis gen. nov., sp. nov. a novel member of the order Bacillales from a saline soil in China. Int J Syst Evol Microbiol 56:1189–1192 [CrossRef]
    [Google Scholar]
  16. Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1980; Isolation of extremely halophilic bacteria able to grow in defined inorganic media with single carbon sources. J Gen Microbiol 119:535–538
    [Google Scholar]
  17. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  18. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Völker H., Thomm M. 2001; Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn–d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51:425–431
    [Google Scholar]
  19. Sehgal S. N., Gibbons N. E. 1960; Effects of metal ions on the growth of Halobacterium cutirubrum . Can J Microbiol 6:165–169 [CrossRef]
    [Google Scholar]
  20. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. 1996; Halobacillus gen. nov., with description of Halobacillus litoralis sp.nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophila comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
    [Google Scholar]
  21. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. 1995; Taxonomic dissection of the genus Micrococcus : Kocuria gen.nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45:682–692 [CrossRef]
    [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  23. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
    [Google Scholar]
  24. Ventosa A., García M. T., Kamekura M., Onishi H., Ruiz-Berraquero F. 1989; Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Syst Appl Microbiol 12:162–166 [CrossRef]
    [Google Scholar]
  25. Ventosa A., Márquez M. C., Ruiz-Berraquero F., Kocur M. 1990; Salinicoccus roseus gen. nov., sp. nov. a new moderately halophilic gram-positive coccus. Syst Appl Microbiol 13:29–33 [CrossRef]
    [Google Scholar]
  26. Ventosa A., Márquez M. C., Garabito M. J., Arahal D. R. 1998a; Moderately halophilic gram-positive bacterial diversity in hypersaline environments. Extremophiles 2:297–304 [CrossRef]
    [Google Scholar]
  27. Ventosa A., Nieto J. J., Oren A. 1998b; Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62:504–544
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  29. Yi H., Schumann P., Sohn K., Chun J. 2004; Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with l-ornithine and l-serine in the peptidoglycan. Int J Syst Evol Microbiol 54:1585–1589 [CrossRef]
    [Google Scholar]
  30. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  31. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  32. Yoon J.-H., Lee K.-C., Weiss N., Kang K. H., Park Y.-H. 2003; Jeotgalicoccus halotolerans gen. nov., sp. nov. and Jeotgalicoccus psychrophilus sp. nov. isolated from the traditional Korean fermented seafood jeotgal. Int J Syst Evol Microbiol 53:595–602 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65003-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65003-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error