1887

Abstract

Strain SH-6 was isolated from the sediment of Lake Shangmatala, a saline lake in Inner Mongolia (China). Cells were pleomorphic. The organism was neutrophilic and required at least 2.5 M (15 %) NaCl, but not MgCl, for growth; optimal growth occurred at 4.3 M (25 %) NaCl. The G+C content of its DNA was 63.1 mol%. 16S rRNA gene sequence analysis revealed that strain SH-6 is a member of the family , but there was a low level of similarity with other members of this family. Highest sequence similarity (94.6 %) was obtained with the 16S rRNA genes of the type strains of and . Polar lipid analyses revealed that strain SH-6 contains phosphatidylglycerol and phosphatidylglyceromethylphosphate, derived from both CC and CC glycerol diethers together with the glycolipid S-DGD-1. On the basis of the data obtained, the new isolate could not be classified in any recognized genus. Strain SH-6 is thus considered to represent a novel species in a new genus within the family , order , for which the name gen. nov., sp. nov. is proposed. The type strain of is SH-6 (=CECT 7173=CGMCC 1.6379=JCM 14033).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65001-0
2007-07-01
2019-08-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1402.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65001-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., Dewhirst, F. E., Paster, B. J., Volcani, B. E. & Ventosa, A. ( 1996; ). Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Appl Environ Microbiol 62, 3779–3786.
    [Google Scholar]
  2. Castillo, A. M., Gutiérrez, M. C., Kamekura, M., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A. ( 2006a; ). Halovivax asiaticus gen. nov., sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. Int J Syst Evol Microbiol 56, 765–770.[CrossRef]
    [Google Scholar]
  3. Castillo, A. M., Gutiérrez, M. C., Kamekura, M., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A. ( 2006b; ). Halostagnicola larsenii gen. nov., sp. nov., an extremely halophilic archaeon from a saline lake in Inner Mongolia, China. Int J Syst Evol Microbiol 56, 1515–1517.[CrossRef]
    [Google Scholar]
  4. Gibbons, N. E. ( 1974; ). Family V. Halobacteriaceae fam. nov. In Bergey's Manual of Determinative Bacteriology, 8th edn, pp. 269–273. Edited by R. E. Buchanan & N. E. Gibbons. Baltimore: Williams & Wilkins.
  5. Gonzalez, C., Gutierrez, C. & Ramírez, C. ( 1978; ). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24, 710–715.[CrossRef]
    [Google Scholar]
  6. Grant, W. D. ( 2001a; ). Genus I. Halobacterium Elazari-Volcani 1957, 207,AL emend. Larsen and Grant 1989, 2222. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 301–305. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  7. Grant, W. D. ( 2001b; ). Genus IV. Halococcus Schoop 1935a, 817AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 311–314. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  8. Grant, W. D., Kamekura, M., McGenity, T. J. & Ventosa, A. ( 2001; ). Order I. Halobacteriales Grant and Larsen 1989b, 495vp (Effective publication: Grant and Larsen 1989a, 2216). In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 294–299. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  9. Hezayen, F. F., Tindall, B. J., Steinbüchel, A. & Rehm, B. H. A. ( 2002; ). Characterization of a novel halophilic archaeon, Halobiforma haloterrestris gen. nov., sp. nov., and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. Int J Syst Bacteriol 52, 2271–2280.[CrossRef]
    [Google Scholar]
  10. Itoh, T., Yamaguchi, T., Zhou, P. & Takashina, T. ( 2005; ). Natronolimnobius baerhuensis gen. nov., sp. nov. and Natronolimnobius innermongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia, China. Extremophiles 9, 111–116.[CrossRef]
    [Google Scholar]
  11. Kamekura, M. ( 1993; ). Lipids of extreme halophiles. In The Biology of Halophilic Bacteria, pp. 135–161. Edited by R. H. Vreeland & L. I. Hochstein. Boca Raton, FL: CRC Press.
  12. Kamekura, M. & Dyall-Smith, M. L. ( 1995; ). Taxonomy of the family Halobacteriaceae and the description of two genera Halorubrobacterium and Natrialba. J Gen Appl Microbiol 41, 333–350.[CrossRef]
    [Google Scholar]
  13. Kamekura, M., Dyall-Smith, M. L., Upasani, V., Ventosa, A. & Kates, M. ( 1997; ). Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum, Natronobacterium magadii, and Natronobacterium pharaonis to Halorubrum, Natrialba and Natronomonas gen. nov., respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively. Int J Syst Bacteriol 47, 853–857.[CrossRef]
    [Google Scholar]
  14. Lopez-Garcia, P., Moreira, D., Lopez-Lopez, A. & Rodríguez-Valera, F. ( 2001; ). A novel haloarchaeal-related lineage is widely distributed in deep oceanic regions. Environ Microbiol 3, 72–78.[CrossRef]
    [Google Scholar]
  15. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  16. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  17. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  18. McGenity, T. J., Gemmell, R. T. & Grant, W. D. ( 1998; ). Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom. nov. and Natrinema pallidum nom. nov. Int J Syst Bacteriol 48, 1187–1196.[CrossRef]
    [Google Scholar]
  19. Montalvo-Rodriguez, R., Vreeland, R. H., Oren, A., Kessel, M., Betancourt, C. & Lopez-Garriga, J. ( 1998; ). Halogeometricum borinquense gen. nov., sp. nov., a novel halophilic archaeon from Puerto Rico. Int J Syst Bacteriol 48, 1305–1312.[CrossRef]
    [Google Scholar]
  20. Oren, A. ( 2006; ). The order Halobacteriales. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 3, pp. 113–164. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer.
  21. Oren, A., Gurevich, P., Gemmell, R. T. & Teske, A. ( 1995; ). Halobaculum gomorrense gen. nov., sp. nov., a novel extremely halophilic archaeon from the Dead Sea. Int J Syst Bacteriol 45, 747–754.[CrossRef]
    [Google Scholar]
  22. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  23. Oren, A., Elevi, R., Watanabe, S., Ihara, K. & Corcelli, A. ( 2002; ). Halomicrobium mukohataei gen. nov., comb. nov., and emended description of Halomicrobium mukohataei. Int J Syst Evol Microbiol 52, 1831–1835.[CrossRef]
    [Google Scholar]
  24. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists, 2nd edn, pp. 217–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Tindall, B. J. ( 1992; ). The family Halobacteriaceae. In The Prokaryotes. A Handbook of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, vol. 1, pp. 768–808. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  27. Tindall, B. J., Ross, H. N. M. & Grant, W. D. ( 1984; ). Natronobacterium gen. nov. and Natronococcus gen. nov., two new genera of haloalkaliphilic archaebacteria. Syst Appl Microbiol 5, 41–57.[CrossRef]
    [Google Scholar]
  28. Torreblanca, M., Rodriguez-Valera, F., Juez, G., Ventosa, A., Kamekura, M. & Kates, M. ( 1986; ). Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition and description of Haloarcula gen. nov. and Haloferax gen. nov. Syst Appl Microbiol 8, 89–99.[CrossRef]
    [Google Scholar]
  29. Ventosa, A. ( 2006; ). Unusual micro-organisms from unusual habitats: hypersaline environments. In Prokaryotic Diversity: Mechanisms and Significance (Society for General Microbiology Symposium no. 66), pp. 223–253. Edited by N. A. Logan, H. M. Lappin-Scott & P. C. F. Oyston. Cambridge: Cambridge University Press.
  30. Ventosa, A., Gutiérrez, M. C., Kamekura, M. & Dyall-Smith, M. L. ( 1999; ). Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49, 131–136.[CrossRef]
    [Google Scholar]
  31. Vreeland, R. H., Straight, S., Krammes, J., Dougherty, K., Rosenzweig, W. D. & Kamekura, M. ( 2002; ). Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles 6, 445–452.[CrossRef]
    [Google Scholar]
  32. Wainø, M., Tindall, B. J. & Ingvorsen, K. ( 2000; ). Halorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah. Int J Syst Evol Microbiol 50, 183–190.[CrossRef]
    [Google Scholar]
  33. Wilson, K. ( 1987; ). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 241–242. Edited by F. M. Ausubel, R. Bent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Wiley.
  34. Xin, H., Itoh, T., Zhou, P., Suzuki, K., Kamekura, M. & Nakase, Y. ( 2000; ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50, 1297–1303.[CrossRef]
    [Google Scholar]
  35. Xu, Y., Zhou, P. & Tian, X. ( 1999; ). Characterization of two novel haloalkaliphilic archaea, Natronorubrum bangense gen. nov., sp. nov. and Natronorubrum tibetense gen. nov., sp. nov. Int J Syst Bacteriol 49, 261–266.[CrossRef]
    [Google Scholar]
  36. Xue, Y., Fan, H., Ventosa, A., Grant, W. D., Jones, B. E., Cowan, D. A. & Ma, Y. ( 2005; ). Halalkalicoccus tibetensis gen. nov., sp. nov., representing a novel genus of haloalkaliphilic archaea. Int J Syst Evol Microbiol 55, 2501–2505.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65001-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65001-0
Loading

Data & Media loading...

vol. , part 7, pp. 1402 - 1407

Thin-layer chromatogram of polar lipids extracted from strain SH-6 and some other haloarchaea. Lanes: 1, sp. A-10; 2, DSM 17691 ; 3, strain SH-6 ; 4, NRC-1. Circled spots are glycolipids. PG, Phosphatidylglycerol; PGP-Me, phosphatidylglycerophosphate methyl ester; S-TGA, sulfated triglycosyl diether; S DGD-1, sulfated diglycosyl diether lipid 1; S-TeGA, sulfated tetraglycosyl diether.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error