1887

Abstract

A Gram-positive, motile, endospore-forming, irregular rod-shaped (0.7–0.9×2.5–5.0 μm), halophilic bacterial strain, NTU-101, was isolated from Chigu saltern in southern Taiwan, previously used as a salt production field. The isolate was characterized taxonomically based on biochemical and molecular approaches. It grows optimally at 40 °C and in the presence of 5–10 % NaCl. Strain NTU-101 has cell-wall peptidoglycan based on -diaminopimelic acid and MK-7 as the predominant menaquinone. Major polar lipids are phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. Anteiso-C, iso-C and anteiso-C are the major fatty acids. The DNA G+C content was 37.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed its affiliation to the genus . DNA–DNA relatedness values between strain NTU-101 and and were 17.5 and 21.5 %, respectively. Based on the phenotypic and phylogenetic properties, strain NTU-101 (=BCRC 17637=CGMCC 1.6496) was classified as a novel strain of species, for which the name sp. nov. is proposed.

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2008-02-01
2020-01-25
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References

  1. An, S. Y., Asahara, M., Goto, K., Kasai, H. & Yokota, A. ( 2007; ). Virgibacillus halophilus sp. nov., spore-forming bacteria isolated from soil in Japan. Int J Syst Evol Microbiol 57, 1607–1611.[CrossRef]
    [Google Scholar]
  2. Anton, J., Oren, A., Benlloch, S., Rodriguez-Valera, F., Amann, R. & Rossello-Mora, R. ( 2002; ). Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52, 485–491.
    [Google Scholar]
  3. Descheemaeker, P. & Swings, J. ( 1995; ). The application of fatty acid methyl ester analysis (FAME) for the identification of heterotrophic bacteria present in decaying Lede-stone of the St. Bavo Cathedral in Ghent. Sci Total Environ 167, 241–247.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Heyndrickx, M., Lebbe, L., Kersters, K., De Vos, P., Forsyth, G. & Logan, N. A. ( 1998; ). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48, 99–106.[CrossRef]
    [Google Scholar]
  6. Heyndrickx, M., Lebbe, L., Kersters, K., Hoste, B., De Wachter, R., De Vos, P., Forsyth, G. & Logan, N. A. ( 1999; ). Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. Int J Syst Bacteriol 49, 1083–1090.[CrossRef]
    [Google Scholar]
  7. Heyrman, J., Mergaert, J., Denys, R. & Swings, J. ( 1999; ). The use of fatty acid methyl ester analysis (FAME) for the identification of heterotrophic bacteria present on three mural paintings showing severe damage by microorganisms. FEMS Microbiol Lett 181, 55–62.[CrossRef]
    [Google Scholar]
  8. Heyrman, J., Logan, N. A., Busse, H.-J., Balcaen, A., Lebbe, L., Rodriguez-Diaz, M., Swings, J. & De Vos, P. ( 2003; ). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53, 501–511.[CrossRef]
    [Google Scholar]
  9. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  10. Lee, J. S., Lim, J. M., Lee, K. C., Lee, J. C., Park, Y. H. & Kim, C. J. ( 2006; ). Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56, 251–257.[CrossRef]
    [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. Quesada, T., Aguilera, M., Morillo, J. A., Ramos-Cormenzana, A. & Monteoliva-Sánchez, M. ( 2007; ). Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives. Int J Syst Evol Microbiol 57, 906–910.[CrossRef]
    [Google Scholar]
  13. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H. ( 1996; ). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69.
    [Google Scholar]
  14. Stackebrandt, E. & Liesack, W. ( 1993; ). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 152–189. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  15. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  16. Yoon, J. H., Oh, T. K. & Park, Y. H. ( 2004; ). Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54, 2163–2167.[CrossRef]
    [Google Scholar]
  17. Yoon, J. H., Kang, S. J., Lee, S. Y., Lee, M. H. & Oh, T. K. ( 2005; ). Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea. Int J Syst Evol Microbiol 55, 1833–1837.[CrossRef]
    [Google Scholar]
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