Indigo-reducing, obligately alkaliphilic strains A11, F11 and F12 were isolated from indigo fermentation liquor obtained from Tokushima Prefecture, Shikoku, Japan. The isolates grew at pH 9.0–12.3, but not at pH 7.0–8.0. The optimum pH range for growth was 9.5–11.5. They were Gram-negative, facultatively anaerobic, rod-shaped strains with peritrichous flagella. The isolates grew in 0–14 % (w/v) NaCl, with optimum growth at 1–11 %. They grew at temperatures of 15–35 °C with optimum growth at around 20–30 °C. -Lactate was the major end product from -glucose. No quinones were detected. The peptidoglycan type was A4, -Lys (-Orn)–-Asp. The major cellular fatty acids were C, C7 and C9. The DNA G+C contents were 47.0–47.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the isolates belong to the genus . DNA–DNA hybridization revealed low relatedness values between the isolates and the three phylogenetically most closely related species, , and (<41 %). On the basis of phenotypic characteristics, including hydrolysis of cellulose and fermentation of carbohydrates, and chemotaxonomic characteristics, phylogenetic data and DNA–DNA relatedness data, it is concluded that the isolates merit classification as representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain of this species is A11 (=JCM 14232=NCIMB 14253).


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vol. , part 4, pp. 901 – 905

Fatty acid profiles of sp. nov. and related species [ PDF] (36 KB)


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