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Abstract

Research at the Center for Biological Defense identified plasmid-borne forms of pXO2 genes in a Gram-positive, endospore-forming rod, isolated from a forensic specimen considered a credible threat of harbouring anthrax. Conventional, commercial and molecular-based methods indicated that the isolate (CBD 119) was not and considered not to be a member of the group. Based on the 16S rRNA gene sequence similarities, strain CBD 119 was most closely related to LMG 18422 (99.3 %). Phenotyping and fatty acid methyl ester analysis of the isolate were conducted alongside JCM 12212. The major cellular fatty acids (anteiso-C, iso-C, and >7 iso or anteiso forms) supported inclusion of the isolate in the genus . Strain CBD 119 was inconsistent with JCM 12212 for 18 of 96 traits evaluated including motility, degree of endospore-driven swelling and pH optimum; the two were linked by fatty acid methyl ester analysis as separate but closely related species. DNA–DNA relatedness between strain CBD 119 and JCM 12212 resulted in less than 20 % hybridization. The results of biochemical and physiological characterization, chemotaxonomic analysis and DNA–DNA hybridization differentiated strain CBD 119 both phenotypically and genotypically from the only species with validly published name with greater than 97 % 16S rRNA gene sequence similarity. The isolate has an accelerated doubling time when grown in aerated broth at pH 5.9 relative to that at pH 7.1. Therefore, it is proposed that strain CBD 119 represents a novel species, sp. nov. The type strain is strain CBD 119 (=NRRL B-41736=DSM 18954).

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2007-09-01
2019-12-11
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vol. , part 9, pp. 2031 - 2036

Specimen source and results of testing by the FDOH, following the LRN Reference Level protocols for identification of

Abridged distance matrix* based on 1340 bp of 16S rRNA gene sequence

Comparison of total fatty acid content between strain CBD 119 and type strain [Single PDF file](124 KB)



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