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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 57, Issue 9

sp. nov., an anaerobic, syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge Free

Abstract

A mesophilic, syntrophic, fatty-acid-oxidizing anaerobic strain, designated MPA, was isolated from granular sludge in a mesophilic upflow anaerobic sludge blanket reactor used to treat palm oil mill effluent. Cells were slightly curved, non-motile rods. Spore formation was not observed. The optimal temperature for growth was around 37 °C and optimal pH for growth was 7.0. Strain MPA was able to grow on crotonate or pentenoate plus butyrate in pure culture. In co-culture with the hydrogenotrophic methanogen , strain MPA was able to oxidize straight-chain saturated fatty acids with carbon chain lengths of C4–C18. The strain was unable to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate, iron(III) or DMSO as an electron acceptor. The G+C content of the DNA was 45.0 mol%. Based on comparative 16S rRNA gene sequence analysis, strain MPA was found to be a member of the genus and was most closely related to the type strains of and (sequence similarities of 94 %). Genetic and phenotypic characteristics demonstrated that strain MPA represents a novel species, for which the name sp. nov. is proposed. The type strain is MPA (=JCM 14374=NBRC 102128=DSM 18709).

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Keyword(s): FAME, fatty acid methyl ester and LCFA, long-chain fatty acid
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2007-09-01
2025-12-13

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Syntrophomonas palmitatica sp. nov., an anaerobic, syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge
Int J Syst Evol Microbiol 57, 2137 (2007); https://doi.org/10.1099/ijs.0.64981-0
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