@article{mbs:/content/journal/ijsem/10.1099/ijs.0.64973-0, author = "Wang, Ya-Nan and Cai, Hua and Chi, Chang-Qiao and Lu, An-Huai and Lin, Xian-Gui and Jiang, Zheng-Feng and Wu, Xiao-Lei", title = "Halomonas shengliensis sp. nov., a moderately halophilic, denitrifying, crude-oil-utilizing bacterium", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2007", volume = "57", number = "6", pages = "1222-1226", doi = "https://doi.org/10.1099/ijs.0.64973-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64973-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A moderately halophilic bacterium, designated strain SL014B-85T, was isolated from a crude-oil-contaminated saline soil from Shengli oilfield, Shandong Province, China. Cells were Gram-negative, aerobic, short rods with lateral flagella. Growth occurred at NaCl concentrations of 0–15 % (optimum 5–15 %), at 10–42 °C (optimum 30 °C) and at pH 8.0–9.0 (optimum pH 8.5). The only respiratory quinone was Q9, and the main cellular fatty acids were C18 : 1 ω7c, C16 : 0 and C19 : 0 cyclo ω8c. The G+C content of the DNA was 66.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B-85T belonged to the genus Halomonas in the Gammaproteobacteria, with highest sequence similarity of 98.1 and 97.8 % to Halomonas alimentaria DSM 15356T and Halomonas ventosae DSM 15911T, respectively. DNA–DNA relatedness values were below 40 % with members of closely related Halomonas species. Results of phenotypic, biochemical and phylogenetic analyses revealed that strain SL014B-85T could be classified as representing a novel species of the genus Halomonas, for which the name Halomonas shengliensis sp. nov. is proposed. The type strain is SL014B-85T (=CGMCC 1.6444T=LMG 23897T).", }