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Abstract

A moderately halophilic bacterium, designated strain SL014B-85, was isolated from a crude-oil-contaminated saline soil from Shengli oilfield, Shandong Province, China. Cells were Gram-negative, aerobic, short rods with lateral flagella. Growth occurred at NaCl concentrations of 0–15 % (optimum 5–15 %), at 10–42 °C (optimum 30 °C) and at pH 8.0–9.0 (optimum pH 8.5). The only respiratory quinone was Q9, and the main cellular fatty acids were C 7, C and C cyclo 8c. The G+C content of the DNA was 66.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B-85 belonged to the genus in the , with highest sequence similarity of 98.1 and 97.8 % to DSM 15356 and DSM 15911, respectively. DNA–DNA relatedness values were below 40 % with members of closely related species. Results of phenotypic, biochemical and phylogenetic analyses revealed that strain SL014B-85 could be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SL014B-85 (=CGMCC 1.6444=LMG 23897).

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2007-06-01
2020-09-25
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